SEC23A 抗体 (Internal Region)
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北京 101111
Quick Overview for SEC23A 抗体 (Internal Region) (ABIN488037)
抗原
See all SEC23A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- Recognizes SEC23A.
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交叉反应 （详细）
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Species reactivity (expected):Human, Rat, Equine (Horse), Canine (Dog) and Bovine.
Species reactivity (tested):Mouse. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-ANRAATTGHVID, from the internal region of the protein sequence according to NP_006355.2. Genename: SEC23A
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anti-Sec23 Homolog A (SEC23A) (AA 1-150) antibody
SEC23A 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sec23 Homolog A (SEC23A) (AA 424-569) antibodySEC23A 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Sec23 Homolog A (SEC23A) (Internal Region) antibodyVerified SEC23A 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Sec23 Homolog A (SEC23A) antibodySEC23A 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Detection Limit Dilution: 1/32000. Western blot: 1-3 μg/mL. Approx 100 kDa band observed in lysates of NIH3T3 (calculatedMW of 86.0 kDa according to Human NP_006355.2 and to Mouse NP_033173.2 ).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- SEC23A (Sec23 Homolog A (SEC23A))
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别名
- SEC23A
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背景
- The regulation of intracellular vesicular trafficking is mediated by specific families of proteins that are involved in vesicular budding, translocation, and fusion with target membranes. The protein trafficking between endoplasmic reticulum (ER) and Golgi apparatus is mediated by two distinct membrane coat complexes, COPI and COPII. Two Coat protein II (COPII) proteins are necessary to generate secretory vesicles at the endoplasmic reticulum. COPI consist of 7 protein subunits includinga, b, b1, d, e, d, z. COPI is also responsible for the retrograde golgi to ER transport f dilysine-tagged proteins. Sec23A is a functional human counter part of the yeast COPII component Sec23p which suggests that it plays similar role in the mammalian protein export from ER. Both Sec 23 isoforms (Sec23A and Sec23B) have similar molecular weight 85 kDa and are co-expressed in many human tissues at varying levels of expression. In yeast, the Sec24p protein is the only COPII component in which two close orthologues have been identified. Sec-23p is a component of coat protein II (COPII) coated vesicles involved in protein export from the endoplasmic reticulum (Mizoguchi et. al., 2000). The COPII is required for the vesicle budding from the endoplasmic reticulum and is consist of two heterodimeric subcomplexes, Sec23p/Sec24p and Sec13p/Sec31p and a small GTPase Ser1p (Higashio et al., 2000). Recent data also suggest that Sec23p/Sec24p complexes are in addition to its role in the shaping up of the vesicle, the Sec23-24p complex may be implicated in cargo selection and trafficking of the amount of cargo with in the cells.Synonyms: Protein transport protein Sec23A, SEC23-related protein A
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基因ID
- 10484
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NCBI登录号
- NP_006355
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UniProt
- Q15436
抗原
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