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- 抗原 See all CDC25C 抗体
- CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))
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适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CDC25C antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- This antibody reacts with CDC25C (60 kDa) on Western blotting.
- 交叉反应 （详细）
- Species reactivity (tested):Human, Mouse and Rat.
- 产品特性
- Synonyms: M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, Cell Division Cycle25C
- 纯化方法
- Protein-A Agarose Chromatography
- 免疫原
- Human CDC25C. Remarks: Hybridoma was established by fusion of Mouse myeloma cell NS-2 with Balb/cmouse splenocyte.
- 克隆位点
- DCS-193
- 亚型
- IgG1
- Top Product
- Discover our top product CDC25C Primary Antibody
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anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) (AA 21-130) antibody
CDC25C 适用: 人 WB, ELISA, IHC (p), IP, RNAi 宿主: 小鼠 Monoclonal 3B11 unconjugated
anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) antibodyCDC25C 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Monoclonal 3E6 unconjugated Recombinant Antibody
anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) (Internal Region) antibodyCDC25C 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) (AA 174-473) antibodyCDC25C 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) (pThr48) antibodyCDC25C 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) (AA 290-340) antibodyCDC25C 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) (pSer216) antibodyCDC25C 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) (Internal Region) antibodyCDC25C 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) (pSer216) antibodyCDC25C 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) antibodyCDC25C 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 1F12 unconjugated
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- 应用备注
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Western Blot: 10 μg/mL for chemiluminescence detection system. Positive Controls: HeLa, A431, ZR75-1, HEp-2, NIH/3T3, PC12. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the Lysisbuffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli’s sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system. (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for the transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at room temperature, or overnight at 4°C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7. 2 containing 1%skimmed milk as suggested in the APPLICATIONS for 1 hour at room temperature. (Theoptimal antibody concentration will depend on the experimental conditions. 8) Wash the membrane with PBS (5 minutes x 6 times). 9) Incubate the membrane with the 1: 10,000 POD-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at room temperature. 10) Wash the membrane with PBS (5 minutes x 6 times). 11) Wipe excess buffer from the membrane, then incubate it with appropriatechemiluminescence reagents for 1 minute. Remove extra reagent from the membrane bydabbing with a paper towel, and seal it in plastic wrap. 12) Expose to an X-ray film in a dark room for 5 minutes. Develop the film as usual. Theconditions for exposure and development may vary. Positive Controls for Western blotting: HeLa, A431, ZR75-1, HEp-2, NIH/3T3, PC12
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 50 % Glycerol without preservatives
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
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Store the antibody undiluted at -20 °C.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))
- 别名
- CDC25C (CDC25C 产品)
- 别名
- CDC25C antibody, cdc25 antibody, xcdc25c antibody, CDC25 antibody, PPP1R60 antibody, Cdc25 antibody, cdc25-3 antibody, xcdc25-3 antibody, cell division cycle 25C antibody, cell division cycle 25C S homeolog antibody, CDC25C antibody, cdc25c antibody, Cdc25c antibody, cdc25c.S antibody
- 背景
- The activity of cyclin-dependent kinases is regulated by their phosphorylation status, which is controlled by the antagonistic action of CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25C is a ~56 kDa dual specific protein phosphatase that controls entry into mitosis by regulating the dephosphorylation of the Cdk1/cyclin B complex. Phosphorylation of Cdc25C creates a binding site for 14-3-3 protein which inhibits Cdc25C. This prevents activation of the Cdk1/cyclin B complex and prevents mitotic entry. Cdc25C is phosphorylated by checkpoint kinases throughout interphase but becomes dephosphorylated during mitosis. Cdc25C is also regulated by p53 via a p53 response element in its promoter, and it is predominantly expressed in the G2 phase of the cell cycle.Synonyms: Cell Division Cycle 25C, Dual specificity phosphatase Cdc25C, M-phase inducer phosphatase 3
- 基因ID
- 995
- UniProt
- P30307
- 途径
- Cell Division Cycle, M Phase
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