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- 抗原 See all CD161 (KLRB1) 抗体
- CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD161 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS)
- 特异性
- This antibody reacts with Human CD161 antigen.
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 产品特性
- Synonyms: HNKR-P1a, CLEC5B, NKRP1A, Killer cell lectin-like receptor subfamily B member 1, Naturalkiller cell surface protein P1A, C-type lectin domain family 5 member B
- 纯化方法
- Protein-A Sepharose Chromatography.
- 免疫原
- Human polyclonal NK cells. Remarks: Hybridoma was established by fusion of mouse myeloma cell SP2 with Balb/cmouse splenocyte.
- 克隆位点
- HP-3G10
- 亚型
- IgG1
- Top Product
- Discover our top product KLRB1 Primary Antibody
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anti-Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1) (AA 73-188) antibody
KLRB1 适用: 人 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1) antibodyKLRB1 适用: 人, Non-Human Primate FACS, WB, IHC (fro) 宿主: 小鼠 Monoclonal HP-3G10 unconjugated
anti-Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1) antibody (PE)KLRB1 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal 10-78 PE
anti-Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1) (AA 67-225) antibodyKLRB1 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 4C6A11 unconjugated
anti-Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1) (AA 151-225) antibodyKLRB1 适用: 人, 大鼠 ELISA 宿主: 兔 Polyclonal unconjugated
anti-Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1) antibodyKLRB1 适用: 大鼠 FACS, WB, IP, IHC (fro), RIA 宿主: 小鼠 Monoclonal 10-78 unconjugated
anti-Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1) antibody (FITC)KLRB1 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal 10-78 FITC
anti-Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1) antibodyKLRB1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1) (AA 126-225) antibodyKLRB1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F3 unconjugated
anti-Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1) antibody (PE)KLRB1 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal HP-3G10 PE
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- 应用备注
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Flow Cytometry: 10-20 μg/mL (final concentration). Positive Control: Lymphocyte cells. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Flow Cytometric analysis for floating cellsProtocol 1We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Resuspend the cells with washing buffer (5x10e6 cells/mL). 2) Add 50 µL of the cell suspension into each tube, and centrifuge at 500xg for 1 minute atroom temperature (20~25°C). Remove supernatant by careful aspiration. 3) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3to the cell pellet after tapping. Mix well, and incubate for 5 minutes at room temperature(20~25°C). 4) Add 30 µL of the CD161 monoclonal antibody (10-20 µg/mL) diluted with the washingbuffer. Mix well, and incubate for 30 minutes at room temperature (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Add 30 µL of secondary antibody (1: 40 FITC conjugated anti-mouse IgG diluted with thewashing buffer. Mix well and incubate for 15 minutes at room temperature (20~25°C). 7) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 8) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Protocol 2We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with PBS containing 25% normal goat serum, 1 mg/mL normalhuman IgG and 0. 1% NaN3 (5x10e6 cells/mL). 3) Add 20 µL of the CD161 monoclonal antibody (50 µg/mL) diluted with the washing bufferinto each tube. 4) Add 50 µL of the cell suspension into each tube. Mix well, and incubate for 30 minutesat room temperature (20~25°C). 5) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 6) Resuspend the cells with 50 µL of the washing buffer. 7) Add 20 µL of secondary antibody (1: 10 FITC conjugated anti-mouse IgG diluted with thewashing buffer into each tube. Mix well and incubate for 15 minutes at room temperature. 8) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). 9) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Flow Cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all step described below. 1) Add 20 µL of the CD161 monoclonal antibody (50 µg/mL) diluted with washing buffer[PBS containing 2% fetal calf serum (FCS) and 0. 1% NaN3] into each tube. 2) Add 50 µL of whole blood into each tube. Mix well, and incubate for 30 minutes at roomtemperature (20~25°C). 3) Add 2 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 4) Add 50 µL of secondary antibody (1: 20 FITC conjugated anti-mouse IgG diluted with thewashing buffer. Mix well and incubate for 15 minutes at room temperature (20~25°C). 5) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective package
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 50 % Glycerol without preservatives.
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
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Store the antibody undiluted at -20 °C.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- CD161 (KLRB1) (Killer Cell Lectin-Like Receptor Subfamily B, Member 1 (KLRB1))
- 别名
- CD161 / KLRB1 (KLRB1 产品)
- 别名
- NKR-P1 antibody, KLRB1 antibody, 4930431A04Rik antibody, Gm4696 antibody, Klrb1g antibody, Klrb6 antibody, Ly-55 antibody, Ly55 antibody, NKR-P1G antibody, Nkrp-1e antibody, Nkrp1g antibody, CD161 antibody, CLEC5B antibody, NKR antibody, NKR-P1A antibody, NKRP1A antibody, hNKR-P1A antibody, Klrb1d antibody, Klrb1f antibody, Nkr-p1e antibody, killer cell lectin like receptor B1 antibody, killer cell lectin-like receptor subfamily B member 1 antibody, killer cell lectin-like receptor subfamily B, member 1 antibody, KLRB1 antibody, Klrb1 antibody
- 背景
- CD161, also known as NKR-P1, is a receptor originally identified on the surface of natural killer cells that is also present on some subpopulations of CD8+T cells. CD161 is often used as an NK cell marker since it is present on both immature and mature NK cells. CD161 appears to mediate NK cell activation and is up-regulated by IL-12. CD161 is expressed as a disulfide homodimer of ~60 kDa while the ligand for CD161 appears to be a carbohydrate structure that includes GalNAc and GlcNAc structures. CD161+ T cells are commonly found in human liver and intestinal epithelium where they may play a role in local immunity.Synonyms: C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, NKRP1A, Natural killer cell surface protein P1A
- 基因ID
- 3820
- UniProt
- Q12918
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