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- 抗原 See all FHL2 抗体
- FHL2 (Four and A Half LIM Domains 2 (FHL2))
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This FHL2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- 特异性
- This antibody reacts with Mouse FHL2.
- 交叉反应 （详细）
- Species reactivity (tested):Human and Mouse.
- 产品特性
- Synonyms: FHL-2, SLIM 3, DRAL, Four and a half LIM domains protein 2, Skeletal muscle LIM-protein 3,LIM domain protein DRAL
- 纯化方法
- Protein-A Sepharose Chromatography.
- 免疫原
- His-FHL2. Remarks: Hybridoma was established by fusion of Mouse myeloma cell SP2/0-Ag4 withBalb/c mouse splenocyte.
- 克隆位点
- 31-10-34
- 亚型
- IgG2a
- Top Product
- Discover our top product FHL2 Primary Antibody
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anti-Four and A Half LIM Domains 2 (FHL2) (AA 1-279) antibody
FHL2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) antibodyFHL2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) (AA 1-279) antibodyFHL2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G3-1A5 unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) (AA 112-233) antibodyFHL2 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) (AA 199-248) antibodyFHL2 适用: 人, 小鼠, 大鼠, 犬, 马, 斑马鱼, 猴, Bat, Hamster, 小鸡 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) antibodyFHL2 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) (C-Term) antibodyFHL2 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) (C-Term) antibodyVerified FHL2 适用: 人, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Four and A Half LIM Domains 2 (FHL2) (AA 182-231) antibodyFHL2 适用: 人, Cow, 豚鼠, 猴 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Western blot: 1-5 μg/mLPositive Control: C2C12Immunoprecipitation: 3 μg/200-300 μL of cell extract. Positive Control: C2C12Immunohistochemistry: 1-5 μg/mLHeat treatment is necessary for Paraffin Embedded Sections. Autoclave, 10 minutes in citrate buffer ( pH 6.5) at 110 °C
- 实验流程
- SDS-PAGE and Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volumes of cold Lysis buffer (50mM Tris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the Lysisbuffer to make an 8 mg/mL solution. 3) Mix the sample with an equal volume of Laemmli's sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 µL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system. (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for specific transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at RT or overnight at 4°C. 7) Incubate the membrane with the anti-FHL2 (11-134) monoclonal antibody (1-5 µg/mL)diluted with 1% skimmed milk (in PBS, pH 7. 2) for 1 hour at RT. 8) Wash the membrane with PBS (5 minutes x 6 times). 9) Incubate the membrane with the 1: 10000 POD-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at RT. 10) Wash the membrane with PBS (5 minutes x 6 times). 11) Wipe excess buffer from the membrane, then incubate it with appropriatechemiluminescence reagents for 1 minute. Remove extra reagent from the membrane bydabbing with a paper towel, and seal it in plastic wrap. 12) Expose to X-ray film in a dark room for 5 minutes. Develop the film as usual. Theconditions for exposure and development may vary. Positive Controls for Western blotting: C2C12Immunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volumes of cold Lysis buffer (50mM Tris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, then
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 50 % Glycerol without preservatives.
- 储存液
- Without preservative
- 储存条件
- -20 °C
- 储存方法
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Store the antibody undiluted at -20 °C.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- FHL2 (Four and A Half LIM Domains 2 (FHL2))
- 别名
- FHL2 / SLIM3 (FHL2 产品)
- 别名
- AAG11 antibody, DRAL antibody, FHL-2 antibody, SLIM-3 antibody, SLIM3 antibody, C76204 antibody, FHL2 antibody, zgc:92428 antibody, zgc:103466 antibody, four and a half LIM domains 2 antibody, four and a half LIM domains 2a antibody, four and a half LIM domains 2b antibody, FHL2 antibody, Fhl2 antibody, fhl2 antibody, fhl2a antibody, fhl2b antibody
- 背景
- Proteins containing LIM domains (which are double zinc finger motifs implicated in protein binding) are important regulators of cell growth, cell differentiation, and remodeling of the cell cytoskeleton. Human Four-and-a-half LIM-only protein 2 (FHL2), also known as DRAL/Slim3 is a 32 kDa protein expressed predominantly in human heart and to a lesser extent in skeletal muscle, testis, and prostate epithelium. Since FHL2 is abundant in heart tissue, it may play a role in the regulation of myofibrillogenesis of heart via LIM-domain binding to focal adhesions. FHL2 has also been identified as a co-activator of the androgen receptor where it promotes androgen receptor transcriptional activity. Stimulation of the Rho signaling pathway induces translocation of FHL2 to the nucleus and subsequent activation of FHL2- and androgen receptor-dependent genes. FHL2 also acts as a trancriptional repressor in muscle cells and is involved in modulation of beta-catenin-dependent transcription of Wnt-responsive genes.Synonyms: DRAL, FHL-2, Four and a half LIM domains protein 2, LIM domain protein DRAL, SLIM 3, Skeletal muscle LIM-protein 3
- 基因ID
- 2274
- UniProt
- Q14192
- 途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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