ITGA7 抗体
Quick Overview for ITGA7 抗体 (ABIN487465)
抗原
See all ITGA7 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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交叉反应 （详细）
- Species reactivity (tested):Mouse.
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纯化方法
- Protein-A Sepharose Chromatography.
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免疫原
- Mouse myoblasts. Remarks: Hybridoma was established by fusion of mouse myeloma cell SP2/0 with Integrinalpha-7 knockout C57/B6 Mouse splenocyte
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亚型
- IgG1
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anti-Integrin, alpha 7 (ITGA7) (AA 247-279), (N-Term) antibody
ITGA7 适用: 人 WB, FACS, IF, IHC (p) 宿主: 兔 Polyclonal RB52834 unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 701-800) antibodyITGA7 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 1149-1181), (C-Term) antibodyITGA7 适用: 人 WB 宿主: 兔 Polyclonal RB52832 unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 478-577) antibodyITGA7 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 8G2 unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 478-577) antibodyITGA7 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F4 unconjugated
anti-Integrin, alpha 7 (ITGA7) (Cleaved-Glu959) antibodyITGA7 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 175-283) antibodyITGA7 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 478-577) antibodyITGA7 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Integrin, alpha 7 (ITGA7) (AA 880-1020) antibodyITGA7 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Integrin, alpha 7 (ITGA7) (N-Term) antibodyITGA7 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry: 10-20 μg/mL (final concentration). Blocking: more than 10 μg/mLPositive Control: C2C12.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Flow Cytometric Analysis for Floating CellsProtocol 1. We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Detach the cells from culture dishes by tripsinization. 2) Stop the trypsinization by adding medium containing 10% fetal calf serum (FCS). 3) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS)and 0. 1% NaN3]. 4) Add 10 µL of normal goat serum to the cell pellet after tapping. Mix well, and incubatefor 5 minutes at room temperature (20~25°C). 5) Add 30 µL of the anti-mouse integrin alpha-7 monoclonal antibody (6A11) (10-20 µg/mL)diluted with the washing buffer. Mix well, and incubate for 30 minutes at room temperature(20~25°C). 6) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 7) Add 30 µL of secondary antibody (1: 40 FITC conjugated anti-mouse IgG diluted with thewashing buffer. Mix well and incubate for 15 minutes at room temperature (20~25°C). 8) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 9) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.2 containing 50 % Glycerol without preservatives.
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
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Store the antibody undiluted at -20 °C.
Shelf life: one year from despatch. -
有效期
- 12 months
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- ITGA7 (Integrin, alpha 7 (ITGA7))
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别名
- Integrin alpha-7 / ITGA7
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背景
- The integrin family of adhesion molecules participate in important cell-cell and cell-extracellular matrix interactions in a diverse range of biological processes. Integrins are heterodimers consisting of an alpha subunit and a beta subunit. Both alpha and beta subunits are transmembrane proteins with large extracellular domains (>100 kDa for alpha subunit and >75 kDa for beta subunit) that interact with extracellular matrix proteins and relatively small cytoplasmic domains (50 amino acids or less, except for the beta-4 subunit) that interact with cytoskeletal proteins. The adhesiveness of integrins is dynamically regulated in response to cytoplasmic signals, termed
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UniProt
- Q61738
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途径
- Integrin Complex
抗原
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