CD3 抗体
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- 抗原 See all CD3 抗体
- CD3
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适用
- 小鼠
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宿主
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仓鼠
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克隆类型
- 单克隆
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标记
- This CD3 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunofluorescence (IF)
- 特异性
- This antibody reacts with Mouse CD3 (epsilon subunit). It is commonly used as a phenotypic marker for Mouse T cells.
- 交叉反应 （详细）
- Species reactivity (tested):Mouse.
- 产品特性
- Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4
- 纯化方法
- Protein A affinity chromatography
- 免疫原
- Mouse BM10-37 cytotoxic T lymphocytes
- 克隆位点
- 145-2C11
- 亚型
- IgG
- Top Product
- Discover our top product CD3 Primary Antibody
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anti-CD3 (CD3) antibody
CD3 适用: 人, 小鼠, 大鼠 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, CyTOF 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, CyTOF 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, Func, CyTOF 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-57 Biotin
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 PerCP
anti-CD3 (CD3) antibody (APC)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 APC
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- 应用备注
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Flow cytometry: 1 μg/mL (million cells). Immunoprecipitation: 1-2 μg/100-500 μg protein in 1 mLcell lysate. Immunohistochemistry on frozen sections. Immunoflourescence. Functional application: Induction of T cell activation, proliferation or apoptosis (dependingon conditions), in vivo T cell depletion.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH ~7.4, 15 mM Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- CD3
- 别名
- CD3 (CD3 产品)
- 背景
- CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
- 基因ID
- 12501
- UniProt
- P22646
- 途径
- TCR Signaling, Ubiquitin Proteasome Pathway
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