CCL4 抗体
Quick Overview for CCL4 抗体 (ABIN480888)
抗原
See all CCL4 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Recognizes Macrophage Inflammatory Protein 1 beta (MIP1 beta). based on ELISA and Western Blot data, <10 % cross-reactivity with mouse MIP-1b, human MIP-1b, human MCP-2 and <5 % cross-reactivity with mouse MIP-1b. <1 % cross-reactivity with other chemokines tested.
-
纯化方法
- Immunoaffinity purified
-
免疫原
- Sf 21-derived Recombinant, human Macrophage Inflammatory Protein 1 beta (MIP1 beta, CCL4).
-
亚型
- IgG
-
-
-
-
应用备注
-
Approved: ELISA (0.5 - 1 μg/mL), IHC (0.5 - 5 μg/mL), Neut (0.3 - 1.5 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in neutralization of bioactivity, ELISA, Western Blot, and Immunohistochemistry. Neutralization: 0.3-1.5 μg/mL in the presence of 0.04 μg/mL of rhMIP-1b, using the BaF/3 hCCR5 transfected cell chemotaxis assay. In chemotaxis assays, this antibody will neutralize the biological activity of human MIP-1b and mouse MIP-1b at a 25-fold higher IgG concentration. Will not neutralize the biological activity of mouse MIP1b. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human MIP-1b. The detection limit is ~0.3 ng/well. Western Blot: 0.1-0.2 μg/mL. The detection limit is 1.5-3.0 ng/lane, under non-reducing and reducing conditions. Immunohistochemistry: 0.5-5 μg/mL in cultured cells or tissue. sections. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, pH 7.2, 40 % glycerol.
-
注意事项
- Avoid repeat freeze-thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-
- CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
-
别名
- CCL4 / SYCA4
-
背景
-
Name/Gene ID: CCL4
Subfamily: Intercrine beta
Family: Intercrine
Synonyms: CCL4, ACT2, Act-2, AT744.1, C-C motif chemokine 4, Chemokine (C-C motif) ligand 4, CC chemokine ligand 4, HC21, LAG1, MIP1B, MIP1B1, LAG-1, SCYA2, Secreted protein G-26, SIS-gamma, MIP-1-beta, MIP-1-beta(1-69), Small-inducible cytokine A4, T-cell activation protein 2, G-26, Lymphocyte-activation gene 1, PAT 744, Protein H400, SCYA4 -
基因ID
- 6351
-
UniProt
- P13236
抗原
-