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CCL4 抗体

This anti-CCL4 antibody is a 山羊 多克隆 antibody detecting CCL4 in WB, ELISA, IHC 和 Neut. Suitable for 人 和 小鼠.
产品编号 ABIN480888
发货至: 中国

Quick Overview for CCL4 抗体 (ABIN480888)

抗原

See all CCL4 抗体
CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

适用

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人, 小鼠

宿主

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山羊

克隆类型

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多克隆

标记

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This CCL4 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Neutralization (Neut)
  • 特异性

    Recognizes Macrophage Inflammatory Protein 1 beta (MIP1 beta). based on ELISA and Western Blot data, <10 % cross-reactivity with mouse MIP-1b, human MIP-1b, human MCP-2 and <5 % cross-reactivity with mouse MIP-1b. <1 % cross-reactivity with other chemokines tested.

    纯化方法

    Immunoaffinity purified

    免疫原

    Sf 21-derived Recombinant, human Macrophage Inflammatory Protein 1 beta (MIP1 beta, CCL4).

    亚型

    IgG
  • 应用备注

    Approved: ELISA (0.5 - 1 μg/mL), IHC (0.5 - 5 μg/mL), Neut (0.3 - 1.5 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: Suitable for use in neutralization of bioactivity, ELISA, Western Blot, and Immunohistochemistry. Neutralization: 0.3-1.5 μg/mL in the presence of 0.04 μg/mL of rhMIP-1b, using the BaF/3 hCCR5 transfected cell chemotaxis assay. In chemotaxis assays, this antibody will neutralize the biological activity of human MIP-1b and mouse MIP-1b at a 25-fold higher IgG concentration. Will not neutralize the biological activity of mouse MIP1b. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human MIP-1b. The detection limit is ~0.3 ng/well. Western Blot: 0.1-0.2 μg/mL. The detection limit is 1.5-3.0 ng/lane, under non-reducing and reducing conditions. Immunohistochemistry: 0.5-5 μg/mL in cultured cells or tissue. sections.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2, 40 % glycerol.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

    别名

    CCL4 / SYCA4

    背景

    Name/Gene ID: CCL4
    Subfamily: Intercrine beta
    Family: Intercrine

    Synonyms: CCL4, ACT2, Act-2, AT744.1, C-C motif chemokine 4, Chemokine (C-C motif) ligand 4, CC chemokine ligand 4, HC21, LAG1, MIP1B, MIP1B1, LAG-1, SCYA2, Secreted protein G-26, SIS-gamma, MIP-1-beta, MIP-1-beta(1-69), Small-inducible cytokine A4, T-cell activation protein 2, G-26, Lymphocyte-activation gene 1, PAT 744, Protein H400, SCYA4

    基因ID

    6351

    UniProt

    P13236
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