SSEA-4 抗体
The 小鼠 单克隆 anti-SSEA-4 antibody is suitable to detect SSEA-4 in samples from 人 和 小鼠. It has been validated for FACS, IHC 和 IHC (fro).
产品编号 ABIN480275
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for SSEA-4 抗体 (ABIN480275)
抗原
SSEA-4
(Thiosulfate Sulfurtransferase 4 (SSEA-4))
适用
人, 小鼠
宿主
小鼠
克隆类型
单克隆
标记
This SSEA-4 antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))
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特异性
- Recognizes a carbohydrate epitope of human SSEA-4. Species cross-reactivity: Mouse.
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纯化方法
- Protein A purified
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免疫原
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Human embryonal carcinoma cell line 2102 Ep.
Type of Immunogen: Cells -
亚型
- IgG3
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应用备注
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Approved: Flo, IHC, IHC-Fr
Usage: Flow Cytometry, Immunohistochemistry. Immunohistochemistry: This antibody has been used with the appropriate secondary reagents at a concentration of 5-10 μg/mL in fixed cells. Cells were fixed with 4 % paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 10 % normal donkey serum and 1 % BSA at room temperature for 45 minutes. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine Red coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. This antibody can also be used in unfixed, shock frozen tissue at a concentration of 10 μg/mL. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
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浓度
- Lot specific
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缓冲液
- Lyophilized from TBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
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- SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))
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别名
- SSEA-4
抗原
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