SSEA-4 抗体
This anti-SSEA-4 antibody is a 小鼠 单克隆 antibody detecting SSEA-4 in FACS, IHC 和 IHC (fro). Suitable for 人 和 小鼠.
Quick Overview for SSEA-4 抗体 (ABIN480275)
抗原
SSEA-4
(Thiosulfate Sulfurtransferase 4 (SSEA-4))
适用
人, 小鼠
宿主
小鼠
克隆类型
单克隆
标记
This SSEA-4 antibody is un-conjugated
应用范围
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))
-
-
特异性
- Recognizes a carbohydrate epitope of human SSEA-4. Species cross-reactivity: Mouse.
-
纯化方法
- Protein A purified
-
免疫原
-
Human embryonal carcinoma cell line 2102 Ep.
Type of Immunogen: Cells -
亚型
- IgG3
-
-
-
-
应用备注
-
Approved: Flo, IHC, IHC-Fr
Usage: Flow Cytometry, Immunohistochemistry. Immunohistochemistry: This antibody has been used with the appropriate secondary reagents at a concentration of 5-10 μg/mL in fixed cells. Cells were fixed with 4 % paraformaldehyde in PBS at room temperature for 20 minutes, then blocked with PBS containing 10 % normal donkey serum and 1 % BSA at room temperature for 45 minutes. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by Rhodamine Red coupled anti-mouse IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. This antibody can also be used in unfixed, shock frozen tissue at a concentration of 10 μg/mL. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
-
浓度
- Lot specific
-
缓冲液
- Lyophilized from TBS, 5 % trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 mL of PBS is used, the antibody concentration will be 500 μg/mL.
-
-
- SSEA-4 (Thiosulfate Sulfurtransferase 4 (SSEA-4))
-
别名
- SSEA-4
抗原
-
You are here:
