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Neurofilament 150, 200kD 抗体

The 小鼠 单克隆 anti-Neurofilament 150, 200kD antibody is suitable to detect Neurofilament 150, 200kD in samples from 人, 小鼠, 兔 和 大鼠. It has been validated for IHC, WB, ELISA, IHC (fro), IHC (p) 和 IP.
产品编号 ABIN479504
发货至: 中国
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Quick Overview for Neurofilament 150, 200kD 抗体 (ABIN479504)

抗原

Neurofilament 150, 200kD

适用

人, 小鼠, 兔, 大鼠

宿主

  • 1
小鼠

克隆类型

  • 1
单克隆

标记

  • 1
非结合性

应用范围

Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
  • 特异性

    Recognizes the 160 alpha 200kD proteins of human neurofilament. Specifically recognizes a non-phosphorylated epitope in the tail domain of NF-M+H. Dephosphorylation of NF-M+H will increase immunoreactivity with this antibody. Species cross-reactivity: human, rat, mouse, bovine, rabbit, hamster and squid.

    纯化方法

    Purified

    免疫原

    Adult rat neurofilament.

    Type of Immunogen: Protein

    亚型

    IgG1 kappa
  • 应用备注

    Approved: ELISA (0.1 - 0.5 μg/mL), IHC, IHC-Fr, IHC-P, IP, WB (0.5 - 1 μg/mL)

    Usage: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, and Western Blot. ELISA: 0.1-0.5 μg/mL. Immunohistochemistry (Bouin's and alcohol-fixed, paraffin-embedded sections and frozen sections): 5-10 μg/mL. To stain, incubate 30-60 minutes at RT or overnight at 4?C. Western Blot: 0.5-1 μg/mL. Immunoprecipitation: 2-5 μg.

    说明

    Target Species of Antibody: Rat

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS, 0.05 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    Neurofilament 150, 200kD

    别名

    Neurofilament 150, 200kD (NEFM/NEFH)

    背景

    This antibody detects: NEFM + NEFH
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