Neurofilament 150, 200kD 抗体
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Quick Overview for Neurofilament 150, 200kD 抗体 (ABIN479504)
抗原
适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes the 160 alpha 200kD proteins of human neurofilament. Specifically recognizes a non-phosphorylated epitope in the tail domain of NF-M+H. Dephosphorylation of NF-M+H will increase immunoreactivity with this antibody. Species cross-reactivity: human, rat, mouse, bovine, rabbit, hamster and squid.
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纯化方法
- Purified
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免疫原
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Adult rat neurofilament.
Type of Immunogen: Protein -
亚型
- IgG1 kappa
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应用备注
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Approved: ELISA (0.1 - 0.5 μg/mL), IHC, IHC-Fr, IHC-P, IP, WB (0.5 - 1 μg/mL)
Usage: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, and Western Blot. ELISA: 0.1-0.5 μg/mL. Immunohistochemistry (Bouin's and alcohol-fixed, paraffin-embedded sections and frozen sections): 5-10 μg/mL. To stain, incubate 30-60 minutes at RT or overnight at 4?C. Western Blot: 0.5-1 μg/mL. Immunoprecipitation: 2-5 μg. -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Neurofilament 150, 200kD
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别名
- Neurofilament 150, 200kD (NEFM/NEFH)
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背景
- This antibody detects: NEFM + NEFH
抗原
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