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Neurofilament 150, 200kD 抗体

适用: 人, 小鼠, 兔, 大鼠 IHC, WB, ELISA, IHC (fro), IHC (p), IP 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN479504
发货至: 中国
  • 抗原
    Neurofilament 150, 200kD
    适用
    人, 小鼠, 兔, 大鼠
    宿主
    • 1
    小鼠
    克隆类型
    • 1
    单克隆
    标记
    • 1
    非结合性
    应用范围
    Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
    特异性
    Recognizes the 160 alpha 200kD proteins of human neurofilament. Specifically recognizes a non-phosphorylated epitope in the tail domain of NF-M+H. Dephosphorylation of NF-M+H will increase immunoreactivity with this antibody. Species cross-reactivity: human, rat, mouse, bovine, rabbit, hamster and squid.
    纯化方法
    Purified
    免疫原
    Adult rat neurofilament.

    Type of Immunogen: Protein
    亚型
    IgG1 kappa
  • 应用备注
    Approved: ELISA (0.1 - 0.5 μg/mL), IHC, IHC-Fr, IHC-P, IP, WB (0.5 - 1 μg/mL)

    Usage: Suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation, and Western Blot. ELISA: 0.1-0.5 μg/mL. Immunohistochemistry (Bouin's and alcohol-fixed, paraffin-embedded sections and frozen sections): 5-10 μg/mL. To stain, incubate 30-60 minutes at RT or overnight at 4?C. Western Blot: 0.5-1 μg/mL. Immunoprecipitation: 2-5 μg.
    说明

    Target Species of Antibody: Rat

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS, 0.05 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原
    Neurofilament 150, 200kD
    别名
    Neurofilament 150, 200kD (NEFM/NEFH)
    背景
    This antibody detects: NEFM + NEFH
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