Proliferation Marker 抗体
适用: 人, 小鼠, 大鼠
IHC, WB, ELISA, IHC (fro), IHC (p), IP
宿主: 小鼠
Monoclonal
unconjugated
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- 抗原
- Proliferation Marker
- 适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- 特异性
- Reacts with a 14-16kD protein as demonstrated by western blot analysis on the Raji cell line. This antigen was detected after 12 h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat.
- 纯化方法
- Protein A purified
- 免疫原
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Spleen cells of a human patient with hairy cell leukemia.
Type of Immunogen: Cells - 亚型
- IgM kappa
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anti-Proliferation Marker antibody
适用: 人 IHC, WB, IF, StM 宿主: 小鼠 Monoclonal JC1 unconjugated
anti-Proliferation Marker antibody适用: 人 IHC, WB, ELISA, IHC (fro), IHC (p), IP 宿主: 小鼠 Monoclonal IPO-38 unconjugated
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- 应用备注
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Approved: ELISA (1:1000), IHC, IHC-Fr, IHC-P, IP, WB (1:50)
Usage: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry frozen and formalin/paraffin). The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells paraformaldehyde fixed, Triton X-100 permeabilized). An increase of 400 % is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide). IPO-38 does not block the binding of the Ki-67 antibody. ELISA: 1:1000. Immunohistochemistry:1:50 to 1:100. Western Blot: 1:50 to 1:100. Positive control: Tonsilar tissue. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- Proliferation Marker
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