Proliferation Marker 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in IHC, WB, ELISA, IHC (fro), IHC (p) 和 IP. Suitable for 人, 小鼠 和 大鼠.
Quick Overview for Proliferation Marker 抗体 (ABIN478915)
抗原
Proliferation Marker
适用
人, 小鼠, 大鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
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特异性
- Reacts with a 14-16kD protein as demonstrated by western blot analysis on the Raji cell line. This antigen was detected after 12 h of PHA-induced activation in the early G1 phase but absent in non-stimulated lymphocytes. Cross reactivity is likely to occur with mouse and rat.
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纯化方法
- Protein A purified
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免疫原
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Spleen cells of a human patient with hairy cell leukemia.
Type of Immunogen: Cells -
亚型
- IgM kappa
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anti-Proliferation Marker antibody
适用: 人 IHC, WB, IF, StM 宿主: 小鼠 Monoclonal JC1 unconjugated
anti-Proliferation Marker antibody适用: 人 IHC, WB, ELISA, IHC (fro), IHC (p), IP 宿主: 小鼠 Monoclonal IPO-38 unconjugated
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应用备注
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Approved: ELISA (1:1000), IHC, IHC-Fr, IHC-P, IP, WB (1:50)
Usage: Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry frozen and formalin/paraffin). The antibody is reported to recognize a nuclear antigen that is present in the cytoplasm and nuclei of proliferating cells paraformaldehyde fixed, Triton X-100 permeabilized). An increase of 400 % is observed in cells in mitosis (determined by analysis of K562 cells synchronized with colcemide). IPO-38 does not block the binding of the Ki-67 antibody. ELISA: 1:1000. Immunohistochemistry:1:50 to 1:100. Western Blot: 1:50 to 1:100. Positive control: Tonsilar tissue. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 0.01 % sodium azide. No stabilizing proteins added.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Proliferation Marker
抗原
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