CCR7 抗体 (Cy7,PE)
Quick Overview for CCR7 抗体 (Cy7,PE) (ABIN477139)
抗原
See all CCR7 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Affinity purified
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免疫原
- Mouse CCR7
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亚型
- IgG2a kappa
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anti-Chemokine (C-C Motif) Receptor 7 (CCR7) (AA 25-59) antibody
CCR7 适用: 人, 小鼠, 大鼠, 绵羊 FACS, WB, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 7 (CCR7) antibodyCCR7 适用: 人 FACS, WB, ELISA 宿主: 小鼠 Monoclonal 4B7B8 unconjugated
anti-Chemokine (C-C Motif) Receptor 7 (CCR7) (AA 11-37), (N-Term) antibodyCCR7 适用: 人, 小鼠 FACS, WB, IF, IHC (p) 宿主: 兔 Polyclonal RB21736 unconjugated
anti-Chemokine (C-C Motif) Receptor 7 (CCR7) (AA 11-37) antibodyCCR7 适用: 人, 小鼠 FACS, WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 7 (CCR7) (AA 1-100) antibodyCCR7 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 7 (CCR7) (AA 332-378) antibodyCCR7 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 7 (CCR7) (C-Term) antibodyCCR7 适用: 人, 猴, Cow, Bat, 马 IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 7 (CCR7) (C-Term) antibodyCCR7 适用: 人, 猴, Cow IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chemokine (C-C Motif) Receptor 7 (CCR7) antibody (APC)CCR7 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal 4B12 APC
anti-Chemokine (C-C Motif) Receptor 7 (CCR7) antibodyCCR7 适用: 小鼠 FACS, IP 宿主: 大鼠 Monoclonal 4B12 unconjugated
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应用备注
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Approved: Flo
Usage: The 4B12 antibody has been tested by flow cytometric analysis of C57BL/6, Balb/C and SJL/J splenocytes and thymocytes. Important: Staining with the 4B12 monoclonal antibody requires different conditions than typically used for surface-antigen staining. Please use the protocol below. Moreover, we have found that staining at 37 °C, rather than 4 °C, results in brighter 4B12 staining, as well as better resolution between positive and negative populations. Please see data for the PE 4B12 (LS-C106090) which demonstrates a comparison of staining at 4 °C and 37 °C. Staining with 4B12 at 37 °C is not expected to interfere with co-staining other antigens, however this should be evaluated for individual experiments.1. Prepare cell suspension as normal and block FcgammaIIIR/FcgammaIIR with 5 μg/million cells purified anti-mouse CD16/32 (LS-C107169) for 15 minutes on ice. If red blood cell lysis is carried out as part of cell preparation, ensure that fixatives are not present in the red blood cell lysis solution as this will eliminate 4B12 staining. Protocol for RBC Lysis of Mouse Spleen.2. Without washing, add 1 μg/million cells 4B12 and incubate in a 37 °C water bath or at 4 °C (please see notes above) for 0.5 hours.3. Wash cells 1X with 3 mL of Flow Cytometry Staining Buffer and decant supernatant.4. Analyze cells on flow cytometer or proceed with secondary staining on ice as normal. Note: Co-staining mouse CCR7 with the 4B12 antibody and the CCR7 ligand CCL19-Fc may be difficult due to different binding conditions required for the antibody versus the ligand, and steric hindrance which may prevent co-staining of 4B12 and CCL19-Fc. Cross-blocking experiments have demonstrated that 4B12 binding is able to prevent the detectable binding of CCL19-Fc, however not the opposite. Furthermore, the correlation between 4B12 and CCL19-Fc staining may be difficult to predict due to the presence of unknown CCL19-Fc receptors in addition to CCR7. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- Aqeous buffer, 0.09 % sodium azide, may contain carrier protein/stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze. Product is photosensitive and should be protected from light.
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储存条件
- 4 °C
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储存方法
- Store at 4°C. Do not freeze. Protect from light.
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- CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))
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别名
- CCR7
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背景
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Name/Gene ID: CCR7
Subfamily: Chemokine
Family: GPCR
Synonyms: CCR7, BLR2, Chemokine (C-C) receptor 7, CD197, C-C CKR-7, CMKBR7, CC chemokine receptor 7, CCR-7, CD197 antigen, CDw197, EBI1, EVI1, MIP-3 beta receptor, C-c chemokine receptor 7, C-C chemokine receptor type 7, CC-CKR-7, Chemokine c-c motif receptor 7 -
基因ID
- 1236
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UniProt
- P32248
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途径
- Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process
抗原
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