DYNC1I1 抗体 (N-Term)
Quick Overview for DYNC1I1 抗体 (N-Term) (ABIN472619)
抗原
See all DYNC1I1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- Recognizes all known forms of the Mr 74kD intermediate chain subunit (IC74) of mammalian cytoplasmic dynein. The antigenic site has been localized to the first 60 amino acids of the amino terminal portion of the molecule (a region conserved in both IC74 genes). Species cross-reactivity: Rat, mouse, bovine, sheep, fish, Drosophila and Xenopus. Does not react with squid.
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纯化方法
- Purified
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免疫原
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Bovine brain cytoplasmic dynein.
Type of Immunogen: Purified protein -
亚型
- IgG2b
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anti-Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1) (AA 1-220) antibody
DYNC1I1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1) antibodyDYNC1I1 适用: 人, 大鼠, 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1) (AA 1-100) antibodyDYNC1I1 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1) (AA 1-100) antibody (FITC)DYNC1I1 适用: 人, 大鼠 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal FITC
anti-Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1) (AA 110-159) antibodyDYNC1I1 适用: 人, 小鼠, 豚鼠, 马, 兔, 犬, Bat, 猴, 小鸡, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1) (AA 21-70) antibodyDYNC1I1 适用: 人, 小鼠, 豚鼠, 马, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1) (N-Term) antibodyDYNC1I1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ICC (1:50 - 1:100), IF, IP, WB (1:1000 - 1:5000)
Usage: Suitable for use in Immunocytochemistry, Western Blot and Immunoprecipitation. Immunocytochemistry: 1:50-1:100. Reacts with cultured MDCK cells, NRR cells, N1E cells and PTK-1 cells. Western Blot: 1:1000-1:5000. Reacts with cultured MDCK cells, PC-12 cells, N1E cells, neurons, glia and other cultured cells. Immunoprecipitation: 10 μL/0.5g tissue. The monoclonal clearly immunoprecipitates the entire dynein complex stoichiometrically from TX-100 or NP-40 lysates (including the 530kD heavy chain, the light intermediate chains and the light chains) from various tissues and cultured cell lines. If detergents such as SDS are used to prepare the lysates the only dynein subunits which are immunoprecipitated are the IC74 subunits (presumably because the dynein complex dissociates), and other contaminating proteins can be found in SDS immunoprecipitates. -
说明
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Target Species of Antibody: Bovine
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.6, 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- DYNC1I1 (Dynein, Cytoplasmic 1, Intermediate Chain 1 (DYNC1I1))
抗原
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