M-Phase phosphoprotein Epitope (LTPLK Or FTPLQ) 抗体
This 小鼠 单克隆 antibody specifically detects M-Phase phosphoprotein Epitope (LTPLK Or FTPLQ) in ICC, IP, ELISA 和 WB. It exhibits reactivity toward 人.
产品编号 ABIN470947
发货至:
中国
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Quick Overview for M-Phase phosphoprotein Epitope (LTPLK Or FTPLQ) 抗体 (ABIN470947)
抗原
M-Phase phosphoprotein Epitope (LTPLK Or FTPLQ)
适用
人
宿主
小鼠
克隆类型
单克隆
应用范围
Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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特异性
- Recognizes a phosphorylated epitope (S/T) P found in phosphoproteins such as MAP2, HSP70, cdc25 and DNA topoisomerase IIa, most of which are phosphorylated at the onset of mitosis. The number of phosphoproteins recognized by MPM-2 varies from species to species and with the cell type. Species cross-reactivity: animals and plants.
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纯化方法
- Protein G purified
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免疫原
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Mitotic human HeLa cell cytosolic lysate.
Type of Immunogen: Cell extract -
亚型
- IgG1
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应用备注
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Approved: ELISA, ICC, IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in Western Blot, Immunocytochemistry, ELISA and Immunoprecipitation. Immunoprecipitation: Reported by an independent laboratory. Positive control: Colcemid treated HeLa cell lysate or A431 mitotic cells fixed with 3.7 % paraformaldehyde. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 40 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- M-Phase phosphoprotein Epitope (LTPLK Or FTPLQ)
抗原
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