Desmoglein 1/2 抗体 (C-Term)
This anti-Desmoglein 1/2 antibody is a 小鼠 单克隆 antibody detecting Desmoglein 1/2 in IF, IHC, WB, IHC (fro) 和 IHC (p). Suitable for Cow, 人, 小鼠 和 大鼠.
Quick Overview for Desmoglein 1/2 抗体 (C-Term) (ABIN470806)
抗原
Desmoglein 1/2 (DSG1/2)
适用
Cow, 人, 小鼠, 大鼠
宿主
小鼠
克隆类型
单克隆
标记
This Desmoglein 1/2 antibody is un-conjugated
应用范围
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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抗原表位
- C-Term
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特异性
- Species (tested so far) Human, bovine, rat, mouse. Recognizes desmoglein 1 + 2 (desmosome-specific cadherins) in desmosome-bearing cells, does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors specifically detected: all epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide reacting: Mr 165000 Desmoglein, 'band 3' polypeptide of the desmosomal complex. Epitope recognized has been localized at the repeating unit domain ('RUD') of the cytoplasmic C-terminal part of the polypeptide. Reactivities on Cultured Cell Lines (tested so far): A-431, MCF-7, TR-146, PLC and various other human epithelial cell lines, bovine BMGE+H, BMGE-H, MDBK.
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纯化方法
- Protein A purified
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免疫原
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Band 3 polypeptide of isolated desmosomes from bovine muzzle epidermis.
Type of Immunogen: Protein -
亚型
- IgG1
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应用备注
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Approved: IF, IHC, IHC-Fr, IHC-P, WB
Usage: Suitable for use in Immunohistochemistry (frozen sections, paraffin-embedded tissue after microwave treatment), Western Blot, Cryo, Paraffin (Microwave), Immunofluorescence microscopy. Immunohistochemistry: 1:10 with PBS, pH 7.4, Incubation Time 1 h at RT, extended with paraffin. -
说明
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Target Species of Antibody: Bovine
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- deionized water
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浓度
- Lot specific
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缓冲液
- Lyophilized.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Desmoglein 1/2 (DSG1/2)
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别名
- Desmoglein 1/2 (DSG1/DSG2)
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背景
- This antibody detects: DSG1+2 / Desmoglein 1+2
抗原
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