Cyclin D1/D2 抗体
适用: 人, Hamster, 小鼠, 大鼠
IP, WB, IHC, IHC (p)
宿主: 小鼠
Monoclonal
unconjugated
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- 抗原
- Cyclin D1/D2
- 适用
- 人, Hamster, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- Recognizes p36 cyclin D1 and cross-reacts with p34 cyclin D2, which has a highly homologous epitope. No cross-reactivity with cyclin A, cyclin B or cyclin D3. Species cross-reactivity: human, mouse, rat and hamster.
- 纯化方法
- Protein A purified
- 免疫原
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Recombinant human Cyclin D1 expressed in E. coli.
Type of Immunogen: Recombinant protein - 亚型
- IgG2a
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- 应用备注
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Approved: IHC, IHC-P, IP, WB
Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 μg immunoprecipitates cyclin D1 and D2 from 500 μg of A431 RIPA lysate. Immunohistochemistry: Reported to detect cyclin D1 and D2 in paraffin-embedded sections using high energy antigen retrieval (10 mM citrate buffer, pH 6.0, microwaved 3-5 minutes, three times). - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.2, 50 % glycerol.
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- Cyclin D1/D2
- 别名
- Cyclin D1,2
- 背景
- This antibody detects: Cyclin D1+D2
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