Cyclin D1/D2 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in IP, WB, IHC 和 IHC (p). Suitable for 人, Hamster, 小鼠 和 大鼠.
Quick Overview for Cyclin D1/D2 抗体 (ABIN470767)
抗原
Cyclin D1/D2
适用
人, Hamster, 小鼠, 大鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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特异性
- Recognizes p36 cyclin D1 and cross-reacts with p34 cyclin D2, which has a highly homologous epitope. No cross-reactivity with cyclin A, cyclin B or cyclin D3. Species cross-reactivity: human, mouse, rat and hamster.
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纯化方法
- Protein A purified
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免疫原
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Recombinant human Cyclin D1 expressed in E. coli.
Type of Immunogen: Recombinant protein -
亚型
- IgG2a
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应用备注
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Approved: IHC, IHC-P, IP, WB
Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 μg immunoprecipitates cyclin D1 and D2 from 500 μg of A431 RIPA lysate. Immunohistochemistry: Reported to detect cyclin D1 and D2 in paraffin-embedded sections using high energy antigen retrieval (10 mM citrate buffer, pH 6.0, microwaved 3-5 minutes, three times). -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 50 % glycerol.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Cyclin D1/D2
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别名
- Cyclin D1,2
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背景
- This antibody detects: Cyclin D1+D2
抗原
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