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Nerve Growth Factor 抗体

This 兔 多克隆 anti-Nerve Growth Factor antibody specifically detects Nerve Growth Factor in WB, ELISA 和 Neut. The antibody is reactive with 人 samples.
产品编号 ABIN465644
发货至: 中国
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Quick Overview for Nerve Growth Factor 抗体 (ABIN465644)

抗原

See all Nerve Growth Factor (NGF) 抗体
Nerve Growth Factor (NGF)

适用

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克隆类型

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多克隆

标记

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This Nerve Growth Factor antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 特异性

    Human beta-NGF

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human beta-NGF.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA (0.5 - 2 μg/mL), Neut (5 - 8 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma. The recommended concentration is 0.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results were achieved without antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human beta-NGF (1 ng/mL), a concentration of 0.05-0.08 μg/mL of this antibody is required. ELISA: To detect Human beta-NGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human beta-NGF (LS-C104735) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human beta-NGF. Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human beta-NGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    Nerve Growth Factor (NGF)

    别名

    NGF

    背景

    Name/Gene ID: NGF
    Family: NGF Beta

    Synonyms: NGF, Beta-nerve growth factor, ProNGF, Beta-NGF, HSAN5, NGFB

    基因ID

    4803

    UniProt

    P01138

    途径

    Regulation of Cell Size
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