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IL-5 抗体

IL5 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN465636
发货至: 中国
  • 抗原 See all IL-5 (IL5) 抗体
    IL-5 (IL5) (Interleukin 5 (IL5))
    适用
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    多克隆
    标记
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    This IL-5 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Neutralization (Neut)
    特异性
    Human IL-5
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human IL-5.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product IL5 Primary Antibody
  • 应用备注
    Approved: ELISA (0.5 - 2 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.25 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-5 (5 ng/mL), a concentration of 0.05-0.15 μg/mL of this antibody is required. ELISA: To detect Human IL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-5 (LS-C104567) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-5. Western Blot: To detect Human IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    IL-5 (IL5) (Interleukin 5 (IL5))
    别名
    IL5 (IL5 产品)
    别名
    EDF antibody, IL-5 antibody, TRF antibody, Il-5 antibody, IL5 antibody, interleukin 5 antibody, IL5 antibody, Il5 antibody
    背景
    Name/Gene ID: IL5
    Family: Hormone

    Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF
    基因ID
    3567
    UniProt
    P05113
    途径
    JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour
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