IL-33 抗体
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北京 101111
Quick Overview for IL-33 抗体 (ABIN465633)
抗原
See all IL-33 (IL33) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human IL-33
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Human IL-33.
Type of Immunogen: Cell extract
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应用备注
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Approved: ELISA (0.5 - 2 μg/mL), SwELISA, WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 μg/mL-0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-33 (1 ng/mL), a concentration of 0.2-0.4 μg/mL of this antibody is required. ELISA: To detect hIL-33 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-33 (LS-C104696) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-33. Western Blot: To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- IL-33 (IL33) (Interleukin 33 (IL33))
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别名
- IL33
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背景
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Name/Gene ID: IL33
Family: Interleukin
Synonyms: IL33, C9orf26, DVS27, DVS27-related protein, IL-1F11, IL-33, Interleukin-33, NFEHEV, NF-HEV, Interleukin-1 family member 11, NFHEV, IL1F11, Interleukin 33, RP11-575C20.2 -
基因ID
- 90865
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UniProt
- O95760
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途径
- Production of Molecular Mediator of Immune Response
抗原
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