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IL-33 抗体

The 兔 多克隆 anti-IL-33 antibody is suitable to detect IL-33 in samples from 人. It has been validated for WB 和 ELISA.
产品编号 ABIN465632
发货至: 中国
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北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for IL-33 抗体 (ABIN465632)

抗原

See all IL-33 (IL33) 抗体
IL-33 (IL33) (Interleukin 33 (IL33))

适用

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宿主

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克隆类型

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多克隆

标记

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This IL-33 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA
  • 特异性

    Human IL-33

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human IL-33.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA (0.5 - 2 μg/mL), SwELISA, WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.125 μg/mL-0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-33 (1 ng/mL), a concentration of 0.2-0.4 μg/mL of this antibody is required. ELISA: To detect hIL-33 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-33 (LS-C104696) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-33. Western Blot: To detect hIL-33 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-33 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    IL-33 (IL33) (Interleukin 33 (IL33))

    别名

    IL33

    背景

    Name/Gene ID: IL33
    Family: Interleukin

    Synonyms: IL33, C9orf26, DVS27, DVS27-related protein, IL-1F11, IL-33, Interleukin-33, NFEHEV, NF-HEV, Interleukin-1 family member 11, NFHEV, IL1F11, Interleukin 33, RP11-575C20.2

    基因ID

    90865

    UniProt

    O95760

    途径

    Production of Molecular Mediator of Immune Response
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