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CCL5 抗体

CCL5 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN465616
发货至: 中国
  • 抗原 See all CCL5 抗体
    CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
    适用
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    宿主
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    克隆类型
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    多克隆
    标记
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    This CCL5 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Neutralization (Neut)
    特异性
    Mouse RANTES
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Murine RANTES.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product CCL5 Primary Antibody
  • 应用备注
    Approved: ELISA (0.5 - 2 μg/mL), Neut (6 - 8 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained colchicine injected mouse brain (somatosensory cortex) tissue. The primary antibody was incubated at 0.5 μg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of mRANTES (100.00 ng/mL), a concentration of 0.8-1.2 μg/mL of this antibody is required. ELISA: To detect mRANTES by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RANTES (LS-C104424) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mRANTES. Western Blot: To detect mRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mRANTES is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Mouse

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    100 μL sterile ddH20
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
    别名
    CCL5 / RANTES (CCL5 产品)
    别名
    Rantes antibody, Scya5 antibody, D17S136E antibody, RANTES antibody, SCYA5 antibody, SIS-delta antibody, SISd antibody, TCP228 antibody, eoCP antibody, MuRantes antibody, C-C motif chemokine 5-like antibody, C-C motif chemokine ligand 5 antibody, chemokine (C-C motif) ligand 5 antibody, LOC100230935 antibody, CCL5 antibody, Ccl5 antibody
    背景
    Name/Gene ID: CCL5
    Family: Intercrine

    Synonyms: CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228
    基因ID
    6352
    UniProt
    P13501
    途径
    Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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