RETNLB 抗体
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北京 101111
Quick Overview for RETNLB 抗体 (ABIN465495)
抗原
See all RETNLB 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Mouse RELMbeta
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Murine RELMbeta.
Type of Immunogen: Cell extract
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应用备注
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Approved: ELISA, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33 ng/mL overnight at 4 °C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100 mM Citric Acid was used. Information and photos are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. ELISA: To detect Murine RELMbeta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMbeta (LS-C104626) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine RELMbeta. Western Blot: To detect Murine RELMbeta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMbeta is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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: "The IL-25-dependent tuft cell circuit driven by intestinal helminths requires macrophage migration inhibitory factor (MIF)." in: Mucosal immunology, (2022) (PubMed).
: "Intestinal epithelial tuft cells initiate type 2 mucosal immunity to helminth parasites." in: Nature, Vol. 529, Issue 7585, pp. 226-30, (2016) (PubMed).
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- RETNLB (Resistin Like beta (RETNLB))
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别名
- FIZZ1 / RELM-Beta
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背景
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Name/Gene ID: RETNLB
Synonyms: RETNLB, CCRG, Fizz1, HXCP2, Found in inflammatory zone 1, Resistin like beta, Resistin-like beta, RELM-beta, RELMb, RELMbeta, RETNL2, FIZZ2, Xcp2 -
基因ID
- 84666
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UniProt
- Q9BQ08
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途径
- Hormone Activity
抗原
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