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RETNLB 抗体

There are 2+ publications for this product available. The 兔 多克隆 anti-RETNLB antibody is suitable to detect RETNLB in samples from 小鼠. It has been validated for WB 和 ELISA.
产品编号 ABIN465495
发货至: 中国
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北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for RETNLB 抗体 (ABIN465495)

抗原

See all RETNLB 抗体
RETNLB (Resistin Like beta (RETNLB))

适用

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小鼠

宿主

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克隆类型

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多克隆

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This RETNLB antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA
  • 特异性

    Mouse RELMbeta

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Murine RELMbeta.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA, WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33 ng/mL overnight at 4 °C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100  mM Citric Acid was used. Information and photos are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. ELISA: To detect Murine RELMbeta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMbeta (LS-C104626) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine RELMbeta. Western Blot: To detect Murine RELMbeta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMbeta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • Varyani, Löser, Filbey, Harcus, Drurey, Poveda, Rasid, White, Smyth, Gerbe, Jay, Maizels: "The IL-25-dependent tuft cell circuit driven by intestinal helminths requires macrophage migration inhibitory factor (MIF)." in: Mucosal immunology, (2022) (PubMed).

    Gerbe, Sidot, Smyth, Ohmoto, Matsumoto, Dardalhon, Cesses, Garnier, Pouzolles, Brulin, Bruschi, Harcus, Zimmermann, Taylor, Maizels, Jay: "Intestinal epithelial tuft cells initiate type 2 mucosal immunity to helminth parasites." in: Nature, Vol. 529, Issue 7585, pp. 226-30, (2016) (PubMed).

  • 抗原

    RETNLB (Resistin Like beta (RETNLB))

    别名

    FIZZ1 / RELM-Beta

    背景

    Name/Gene ID: RETNLB

    Synonyms: RETNLB, CCRG, Fizz1, HXCP2, Found in inflammatory zone 1, Resistin like beta, Resistin-like beta, RELM-beta, RELMb, RELMbeta, RETNL2, FIZZ2, Xcp2

    基因ID

    84666

    UniProt

    Q9BQ08

    途径

    Hormone Activity
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