RELM alpha 抗体
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北京 101111
Quick Overview for RELM alpha 抗体 (ABIN465191)
抗原
See all RELM alpha 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Mouse RELMalpha
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Murine RELMalpha.
Type of Immunogen: Cell extract
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应用备注
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Approved: ELISA, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of murine cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse). The primary antibody was incubated at 4 ng/mL overnight at 4 °C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100 mM Citric Acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: To detect Murine RELMalpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMalpha (LS-C104489) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine RELMalpha. Western Blot: To detect Murine RELMalpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMalpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in sterile water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- RELM alpha
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别名
- Fizz1 / RELM Alpha
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背景
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Name/Gene ID: Retnla
Synonyms: Retnla, Fizz-1, Fizz1, HIMF, RELM Alpha, RELM-alpha, RELMa, RELMalpha, Resistin like alpha, Xcp2 -
基因ID
- 57262
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UniProt
- Q9EP95
抗原
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