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RELM alpha 抗体

The 兔 多克隆 anti-RELM alpha antibody is suitable to detect RELM alpha in samples from 小鼠. It has been validated for WB 和 ELISA.
产品编号 ABIN465191
发货至: 中国
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Quick Overview for RELM alpha 抗体 (ABIN465191)

抗原

See all RELM alpha 抗体
RELM alpha

适用

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小鼠

宿主

  • 33
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克隆类型

  • 33
  • 3
多克隆

标记

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This RELM alpha antibody is un-conjugated

应用范围

  • 36
  • 17
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Western Blotting (WB), ELISA
  • 特异性

    Mouse RELMalpha

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Murine RELMalpha.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA, WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of murine cecum tissue (with positive cells infiltrating the Lamina Propia of a Trichuris muris infected mouse). The primary antibody was incubated at 4 ng/mL overnight at 4 °C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100  mM Citric Acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: To detect Murine RELMalpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMalpha (LS-C104489) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine RELMalpha. Western Blot: To detect Murine RELMalpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMalpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute in sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    RELM alpha

    别名

    Fizz1 / RELM Alpha

    背景

    Name/Gene ID: Retnla

    Synonyms: Retnla, Fizz-1, Fizz1, HIMF, RELM Alpha, RELM-alpha, RELMa, RELMalpha, Resistin like alpha, Xcp2

    基因ID

    57262

    UniProt

    Q9EP95
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