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CCL5 抗体

CCL5 适用: 人 Neut 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN465158
发货至: 中国
  • 抗原 See all CCL5 抗体
    CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
    适用
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    宿主
    • 106
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    小鼠
    克隆类型
    • 99
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    单克隆
    标记
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    This CCL5 antibody is un-conjugated
    应用范围
    • 96
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    Neutralization (Neut)
    特异性
    Human RANTES
    纯化方法
    Protein A purified
    免疫原
    E.coli derived recombinant Human RANTES.

    Type of Immunogen: Cell extract
    亚型
    IgG2b
    Top Product
    Discover our top product CCL5 Primary Antibody
  • 应用备注
    Approved: Neut (5 - 7 μg/mL)

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 citrate buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human RANTES (100 ng/mL), a concentration of 3-5 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 500 pg/mL of recombinant human RANTES when used with biotinylated antigen affinity purified anti-human RANTES (LS-C104720) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.5-1 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 0.25-2 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
    别名
    CCL5 / RANTES (CCL5 产品)
    别名
    Rantes antibody, Scya5 antibody, D17S136E antibody, RANTES antibody, SCYA5 antibody, SIS-delta antibody, SISd antibody, TCP228 antibody, eoCP antibody, MuRantes antibody, C-C motif chemokine 5-like antibody, C-C motif chemokine ligand 5 antibody, chemokine (C-C motif) ligand 5 antibody, LOC100230935 antibody, CCL5 antibody, Ccl5 antibody
    背景
    Name/Gene ID: CCL5
    Family: Intercrine

    Synonyms: CCL5, Chemokine (C-C motif) ligand 5, D17S136E, EoCP, T-cell specific protein p288, SCYA5, SISd, T cell-specific protein P228, Beta-chemokine RANTES, C-C motif chemokine 5, RANTES, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, TCP228
    基因ID
    6352
    UniProt
    P13501
    途径
    Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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