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PF4 抗体

PF4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN465152
发货至: 中国
  • 抗原 See all PF4 抗体
    PF4 (Platelet Factor 4 (PF4))
    适用
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    宿主
    • 65
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    克隆类型
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    多克隆
    标记
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    This PF4 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA
    特异性
    Human PF-4
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human PF-4.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product PF4 Primary Antibody
  • 应用备注
    Approved: ELISA (0.5 - 2 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1 mg/mL-2 mg/mL with an overnight incubation at 4 °C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: To detect hPF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PF-4 (LS-C104446) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    PF4 (Platelet Factor 4 (PF4))
    别名
    CXCL4 / PF4 (PF4 产品)
    别名
    CXCL4 antibody, PF-4 antibody, SCYB4 antibody, Cxcl4 antibody, Scyb4 antibody, Pf4a antibody, RATPF4A antibody, PF4A antibody, CXCL4L1 antibody, CXCL4V1 antibody, PF4-ALT antibody, SCYB4V1 antibody, PF4 antibody, platelet factor 4 antibody, platelet factor 4 variant 1 antibody, PF4 antibody, Pf4 antibody, LOC703451 antibody, PF4V1 antibody, LOC100524373 antibody, LOC102181854 antibody, LOC100630489 antibody
    背景
    Name/Gene ID: PF4
    Subfamily: Intercrine alpha
    Family: Intercrine

    Synonyms: PF4, C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4, Platelet factor 4
    基因ID
    5196
    UniProt
    P02776
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