PF4 抗体
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- 抗原 See all PF4 抗体
- PF4 (Platelet Factor 4 (PF4))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This PF4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- Human PF-4
- 纯化方法
- Immunoaffinity purified
- 免疫原
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E.coli derived recombinant Human PF-4.
Type of Immunogen: Cell extract - Top Product
- Discover our top product PF4 Primary Antibody
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- 应用备注
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Approved: ELISA (0.5 - 2 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 1 mg/mL-2 mg/mL with an overnight incubation at 4 °C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. ELISA: To detect hPF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PF-4 (LS-C104446) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Sterile water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- 抗原
- PF4 (Platelet Factor 4 (PF4))
- 别名
- CXCL4 / PF4 (PF4 产品)
- 别名
- CXCL4 antibody, PF-4 antibody, SCYB4 antibody, Cxcl4 antibody, Scyb4 antibody, Pf4a antibody, RATPF4A antibody, PF4A antibody, CXCL4L1 antibody, CXCL4V1 antibody, PF4-ALT antibody, SCYB4V1 antibody, PF4 antibody, platelet factor 4 antibody, platelet factor 4 variant 1 antibody, PF4 antibody, Pf4 antibody, LOC703451 antibody, PF4V1 antibody, LOC100524373 antibody, LOC102181854 antibody, LOC100630489 antibody
- 背景
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Name/Gene ID: PF4
Subfamily: Intercrine alpha
Family: Intercrine
Synonyms: PF4, C-X-C motif chemokine 4, CXCL4, Iroplact, Oncostatin-A, PF-4, SCYB4, Platelet factor 4 - 基因ID
- 5196
- UniProt
- P02776
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