IL-11 抗体
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北京 101111
Quick Overview for IL-11 抗体 (ABIN465009)
抗原
See all IL-11 (IL11) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human IL-11
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Human IL-11.
Type of Immunogen: Cell extract
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应用备注
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Approved: ELISA (0.5 - 2 μg/mL), Neut (0.15 - 0.25 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 mg/mL overnight at 4 °C. This was followed by a fluorophore conjugated secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-11 (1.50 ng/mL), a concentration of 0.02-0.05 μg/mL of this antibody is required. ELISA: To detect hIL-11 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-11 (LS-C104365) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-11. Western Blot: To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile water
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- IL-11 (IL11) (Interleukin 11 (IL11))
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别名
- IL11
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背景
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Name/Gene ID: IL11
Family: Hormone
Synonyms: IL11, AGIF, Adipogenesis inhibitory factor, IL-11, Interleukin-11, Oprelvekin, Interleukin 11 -
基因ID
- 3589
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UniProt
- P20809
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途径
- JAK/STAT Signaling, Negative Regulation of Hormone Secretion
抗原
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