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IL-4 抗体 (Biotin)

The 兔 多克隆 anti-IL-4 antibody is suitable to detect IL-4 in samples from 人. It has been validated for WB 和 ELISA.
产品编号 ABIN464882
发货至: 中国
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北京 101111
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Quick Overview for IL-4 抗体 (Biotin) (ABIN464882)

抗原

See all IL-4 (IL4) 抗体
IL-4 (IL4) (Interleukin 4 (IL4))

适用

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克隆类型

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多克隆

标记

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This IL-4 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA
  • 特异性

    Human IL-4

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human IL-4.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: ELISA: To detect hIL-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-4. To detect hIL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-4 (LS-B7474) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-4. Western Blot: To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile PBS containing 0.1 % BSA

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    IL-4 (IL4) (Interleukin 4 (IL4))

    别名

    IL4

    背景

    Name/Gene ID: IL4
    Family: Hormone

    Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4

    基因ID

    3565

    UniProt

    P05112

    途径

    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
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