MIA 抗体
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北京 101111
Quick Overview for MIA 抗体 (ABIN464851)
抗原
See all MIA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human MIA
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Human MIA.
Type of Immunogen: Cell extract
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anti-Melanoma Inhibitory Activity (MIA) (Internal Region) antibody
Verified MIA 适用: 人 ELISA, IHC, WB 宿主: 山羊 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) (AA 87-124) antibodyMIA 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) antibodyMIA 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) antibodyMIA 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) (AA 36-127) antibodyMIA 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Inhibitory Activity (MIA) (AA 1-131) antibodyMIA 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3A6 unconjugated
anti-Melanoma Inhibitory Activity (MIA) (C-Term) antibodyMIA 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 1 μg/mL-2 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6 Sodium Citrate buffer or enzyme induced antigen retrieval with proteinase K at room temperature. Optimal concentrations and conditions may vary. ELISA: To detect hMIA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIA (LS-C104284) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIA. Western Blot: To detect hMIA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.2
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- MIA (Melanoma Inhibitory Activity (MIA))
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别名
- MIA
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背景
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Name/Gene ID: MIA
Family: MIA
Synonyms: MIA, CD-RAP, Melanoma inhibitory activity -
基因ID
- 8190
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UniProt
- Q16674
抗原
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