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MIA 抗体

MIA 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN464851
发货至: 中国
  • 抗原 See all MIA 抗体
    MIA (Melanoma Inhibitory Activity (MIA))
    适用
    • 31
    • 12
    • 7
    宿主
    • 32
    • 2
    • 1
    克隆类型
    • 33
    • 2
    多克隆
    标记
    • 17
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MIA antibody is un-conjugated
    应用范围
    • 20
    • 16
    • 9
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB)
    特异性
    Human MIA
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human MIA.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product MIA Primary Antibody
  • 应用备注
    Approved: ELISA, WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 1 μg/mL-2 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a  pH 6 Sodium Citrate buffer or enzyme induced antigen retrieval with proteinase K at room temperature. Optimal concentrations and conditions may vary. ELISA: To detect hMIA by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIA (LS-C104284) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIA. Western Blot: To detect hMIA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    MIA (Melanoma Inhibitory Activity (MIA))
    别名
    MIA (MIA 产品)
    别名
    CD-RAP antibody, Mia1 antibody, Cdrap antibody, zgc:194157 antibody, mia antibody, CDRAP antibody, Fdp antibody, MIA antibody, MIAL antibody, melanoma inhibitory activity antibody, otoraplin antibody, cilia and flagella associated protein 100 antibody, MIA antibody, Mia antibody, mia antibody, Otor antibody, CFAP100 antibody
    背景
    Name/Gene ID: MIA
    Family: MIA

    Synonyms: MIA, CD-RAP, Melanoma inhibitory activity
    基因ID
    8190
    UniProt
    Q16674
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