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CCL15 抗体

This anti-CCL15 antibody is a 山羊 多克隆 antibody detecting CCL15 in ELISA, WB 和 Neut. Suitable for 人.
产品编号 ABIN464833
发货至: 中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for CCL15 抗体 (ABIN464833)

抗原

See all CCL15 抗体
CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

适用

宿主

  • 32
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山羊

克隆类型

  • 40
  • 6
多克隆

标记

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This CCL15 antibody is un-conjugated

应用范围

  • 30
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ELISA, Western Blotting (WB), Neutralization (Neut)
  • 特异性

    Human MIP-5

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human MIP-5.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA (0.5 - 2 μg/mL), Neut (1.75 - 3 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hMIP-5 (50 ng/mL), a concentration of 1.75-3 μg/mL of this antibody is required. ELISA: To detect hMIP-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIP-5 (LS-C104743) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIP-5. Western Blot: To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

    别名

    CCL15 / MIP5

    背景

    Name/Gene ID: CCL15
    Subfamily: Intercrine beta
    Family: Intercrine

    Synonyms: CCL15, C-C motif chemokine 15, Chemokine hcc2, HMRP-2B, Leukotactin-1, LKN1, MIP-5, MIP5, MRP-2B, Leukotactin1, Small-inducible cytokine A15, MIP-1D, NCC3, New CC chemokine 3, SY15, Chemokine CC-2, HCC-2, Leukotactin 1, LKN-1, MIP-1 delta, NCC-3, SCYA15

    基因ID

    6359

    UniProt

    Q16663
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