电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

BRCC3 抗体 (AA 1-316)

This anti-BRCC3 antibody is a 小鼠 单克隆 antibody detecting BRCC3 in WB, ELISA, IHC 和 IHC (p). Suitable for 人.
产品编号 ABIN462274
发货至: 中国

Quick Overview for BRCC3 抗体 (AA 1-316) (ABIN462274)

抗原

See all BRCC3 抗体
BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

适用

  • 67
  • 19
  • 18
  • 5
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1

宿主

  • 61
  • 6
小鼠

克隆类型

  • 52
  • 15
单克隆

标记

  • 30
  • 5
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This BRCC3 antibody is un-conjugated

应用范围

  • 54
  • 20
  • 16
  • 13
  • 13
  • 12
  • 8
  • 7
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

AT3B1
  • 抗原表位

    • 15
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-316

    特异性

    BRCC36 (1-316aa)

    纯化方法

    Protein G purified

    免疫原

    Recombinant BRCC36 (1-316aa) purified from E. coli

    Type of Immunogen: Recombinant protein

    亚型

    IgG1 kappa
  • 应用备注

    Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of HeLa(20 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

    别名

    BRCC3 / BRCC36

    背景

    Name/Gene ID: BRCC3

    Synonyms: BRCC3, C6.1A, BRCC36, BRISC complex subunit BRCC36, BRCA1-A complex subunit BRCC36, CXorf53, RP11-143H17.2

    基因ID

    79184

    UniProt

    P46736

    途径

    Positive Regulation of Response to DNA Damage Stimulus
You are here:
Chat with us!