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Glucose-6-Phosphate Dehydrogenase 抗体 (AA 35-506)

This anti-Glucose-6-Phosphate Dehydrogenase antibody is a 小鼠 单克隆 antibody detecting Glucose-6-Phosphate Dehydrogenase in WB, IHC, ELISA 和 IHC (p). Suitable for 人.
产品编号 ABIN462273
发货至: 中国

Quick Overview for Glucose-6-Phosphate Dehydrogenase 抗体 (AA 35-506) (ABIN462273)

抗原

See all Glucose-6-Phosphate Dehydrogenase (G6PD) 抗体
Glucose-6-Phosphate Dehydrogenase (G6PD)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

AT2F6
  • 抗原表位

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    AA 35-506

    特异性

    Human G6PD (35-506aa)

    纯化方法

    Protein G purified

    免疫原

    Recombinant human G6PD (35-506aa) purified from E. coli

    Type of Immunogen: Recombinant protein

    亚型

    IgG2b kappa
  • 应用备注

    Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of MCF7 (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human G6PD (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.1 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    Glucose-6-Phosphate Dehydrogenase (G6PD)

    别名

    G6PD

    背景

    Name/Gene ID: G6PD

    Synonyms: G6PD, G6PD1

    基因ID

    2539

    UniProt

    P11413

    途径

    Regulation of Systemic Arterial Blood Pressure by Hormones
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