Glucose-6-Phosphate Dehydrogenase 抗体 (AA 35-506)
Quick Overview for Glucose-6-Phosphate Dehydrogenase 抗体 (AA 35-506) (ABIN462273)
抗原
See all Glucose-6-Phosphate Dehydrogenase (G6PD) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 35-506
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特异性
- Human G6PD (35-506aa)
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纯化方法
- Protein G purified
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免疫原
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Recombinant human G6PD (35-506aa) purified from E. coli
Type of Immunogen: Recombinant protein -
亚型
- IgG2b kappa
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应用备注
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Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Cell lysates of MCF7 (30 μg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human G6PD (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.1 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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- Glucose-6-Phosphate Dehydrogenase (G6PD)
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别名
- G6PD
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背景
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Name/Gene ID: G6PD
Synonyms: G6PD, G6PD1 -
基因ID
- 2539
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UniProt
- P11413
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones
抗原
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