Tyrosine Hydroxylase 抗体
Quick Overview for Tyrosine Hydroxylase 抗体 (ABIN462131)
抗原
See all Tyrosine Hydroxylase (TH) 抗体适用
宿主
克隆类型
标记
应用范围
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品牌
- IHC-plus™
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特异性
- Recognizes tyrosine hydroxylase. Species cross-reactivity: Mouse, human, ferret, feline and Aplysia (marine mollusc). Because Tyrosine Hydroxylase is highly conserved, this antibody is expected to cross-react with most mammalian species and many non-mammalian species.
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纯化方法
- Immunoaffinity purified
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免疫原
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Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).
Type of Immunogen: Purified protein -
亚型
- IgG
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应用备注
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Approved: ELISA, IF (1:100 - 1:1000), IHC, IHC-Fr, IHC-P (1:100), IP, WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. Western Blot: 1:1000 on 10 μg of PC12 lysates. Immunofluorescence: 1:100-1:1000. Fixed with 4 % PFA overnight at 4C. -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Tyrosine Hydroxylase (TH)
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别名
- TH / Tyrosine Hydroxylase
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背景
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Name/Gene ID: TH
Family: Monooxygenase
Synonyms: TH, DYT14, Dystonia 14, TYH, Tyrosine 3-hydroxylase, Tyrosine hydroxylase, DYT5b, Tyrosine 3-monooxygenase -
基因ID
- 7054
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UniProt
- P07101
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途径
- Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour
抗原
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