RAB3A 抗体 (AA 202-217)
Quick Overview for RAB3A 抗体 (AA 202-217) (ABIN462094)
抗原
See all RAB3A 抗体适用
宿主
克隆类型
标记
应用范围
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                                            抗原表位
- AA 202-217
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                                            品牌
- IHC-plus™
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                                            特异性
- Detects an ~25 kD protein, corresponding to the apparent molecular mass of Rab3A on SDSPAGE immunoblots, in samples from mouse and rat origins. The antibody specificity is confirmed in peptide inhibition immunoblotting studies.
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                                            预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).
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                                            纯化方法
- Immunoaffinity purified
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                                            免疫原
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                        A 16 residue synthetic peptide (C)KQGPQLSDQQVPPHQD based on the human Rab3A (residues 202-217) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).
 Type of Immunogen: Synthetic peptide - KLH conjugated
 
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                                            应用备注
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                        Approved: IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)
 Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.
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                                            说明
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                        Target Species of Antibody: Human 
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                                            限制
- 仅限研究用
 
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                                            状态
- Liquid
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                                            浓度
- Lot specific
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                                            缓冲液
- Borate buffered saline, pH 8.2 (25 mM sodium borate, 100 mM boric acid, 75 mM NaCl, 5 mM EDTA (disodium salt)
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                                            注意事项
- avoid freeze thaw cycles.
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                                            储存条件
- 4 °C,-20 °C
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                                            储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
 
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    - RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))
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                                            别名
- RAB3A
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                                            背景
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                        Name/Gene ID: RAB3A
 Subfamily: RAS oncogene
 Family: Ras GTPase superfamily IPR001806
 Synonyms: RAB3A, RAS-associated protein RAB3A, Ras-related protein Rab-3A
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                                            基因ID
- 5864
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                                            UniProt
- P20336
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                                            途径
- Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
 抗原
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