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RAB3A 抗体 (AA 202-217)

This anti-RAB3A antibody is a 兔 多克隆 antibody detecting RAB3A in WB, IHC 和 IHC (p). Suitable for 人 和 猴.
产品编号 ABIN462094
发货至: 中国

Quick Overview for RAB3A 抗体 (AA 202-217) (ABIN462094)

抗原

See all RAB3A 抗体
RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

适用

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人, 猴

宿主

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克隆类型

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多克隆

标记

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This RAB3A antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    AA 202-217

    品牌

    IHC-plus™

    特异性

    Detects an ~25 kD protein, corresponding to the apparent molecular mass of Rab3A on SDSPAGE immunoblots, in samples from mouse and rat origins. The antibody specificity is confirmed in peptide inhibition immunoblotting studies.

    预测反应

    Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).

    纯化方法

    Immunoaffinity purified

    免疫原

    A 16 residue synthetic peptide (C)KQGPQLSDQQVPPHQD based on the human Rab3A (residues 202-217) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).

    Type of Immunogen: Synthetic peptide - KLH conjugated
  • 应用备注

    Approved: IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Borate buffered saline,  pH 8.2 (25  mM sodium borate, 100  mM boric acid, 75  mM NaCl, 5  mM EDTA (disodium salt)

    注意事项

    avoid freeze thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原

    RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

    别名

    RAB3A

    背景

    Name/Gene ID: RAB3A
    Subfamily: RAS oncogene
    Family: Ras GTPase superfamily IPR001806

    Synonyms: RAB3A, RAS-associated protein RAB3A, Ras-related protein Rab-3A

    基因ID

    5864

    UniProt

    P20336

    途径

    Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
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