SRSF1 抗体 (N-Term)
Quick Overview for SRSF1 抗体 (N-Term) (ABIN461956)
抗原
See all SRSF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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品牌
- IHC-plus™
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特异性
- Specific for the N-terminal portion (aa1-97) of the SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. On Western blots, a band of ~33kD is seen with doublet or triplet bands sometimes evident due to phosphorylation differences. SF2/ASF is highly conserved (e. g. mouse and human protein are 100 % identical) so reactivity with other species is likely. Please note, however, that cross-reactivity in non-target species varies and cannot be guaranteed.
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纯化方法
- Protein A purified
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免疫原
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Recombinant SF2/ASF protein.
Type of Immunogen: Recombinant protein -
亚型
- IgG1
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应用备注
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Approved: IF, IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-3 μg/mL. A band of ~33kD is seen with doublet or triplet bands sometimes evident due to phosphorylation differences. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.1 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))
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别名
- SFRS1 / SF2
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背景
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Name/Gene ID: SRSF1
Synonyms: SRSF1, Alternative-splicing factor 1, ASF, SF2, SF2p33, SFRS1, Splicing factor 2, ASF-1, SR splicing factor 1, SRp30a -
基因ID
- 6426
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UniProt
- Q07955
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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