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SRSF1 抗体 (N-Term)

This anti-SRSF1 antibody is a 小鼠 单克隆 antibody detecting SRSF1 in WB, IHC, IF 和 IHC (p). Suitable for 人, 小鼠 和 大鼠.
产品编号 ABIN461956
发货至: 中国

Quick Overview for SRSF1 抗体 (N-Term) (ABIN461956)

抗原

See all SRSF1 抗体
SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

适用

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人, 小鼠, 大鼠

宿主

  • 53
  • 2
小鼠

克隆类型

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  • 11
单克隆

标记

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This SRSF1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    N-Term

    品牌

    IHC-plus™

    特异性

    Specific for the N-terminal portion (aa1-97) of the SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. On Western blots, a band of ~33kD is seen with doublet or triplet bands sometimes evident due to phosphorylation differences. SF2/ASF is highly conserved (e. g. mouse and human protein are 100 % identical) so reactivity with other species is likely. Please note, however, that cross-reactivity in non-target species varies and cannot be guaranteed.

    纯化方法

    Protein A purified

    免疫原

    Recombinant SF2/ASF protein.

    Type of Immunogen: Recombinant protein

    亚型

    IgG1
  • 应用备注

    Approved: IF, IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-3 μg/mL. A band of ~33kD is seen with doublet or triplet bands sometimes evident due to phosphorylation differences.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.1 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    avoid freeze thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
  • 抗原

    SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

    别名

    SFRS1 / SF2

    背景

    Name/Gene ID: SRSF1

    Synonyms: SRSF1, Alternative-splicing factor 1, ASF, SF2, SF2p33, SFRS1, Splicing factor 2, ASF-1, SR splicing factor 1, SRp30a

    基因ID

    6426

    UniProt

    Q07955

    途径

    Ribonucleoprotein Complex Subunit Organization
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