Nuclease 抗体
This anti-Nuclease antibody is a 兔 多克隆 antibody detecting Nuclease in ELISA, WB 和 IF. Suitable for Staphylococcus aureus.
Quick Overview for Nuclease 抗体 (ABIN459242)
抗原
Nuclease
适用
Staphylococcus aureus
宿主
兔
克隆类型
多克隆
标记
This Nuclease antibody is un-conjugated
应用范围
ELISA, Western Blotting (WB), Immunofluorescence (IF)
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特异性
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
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产品特性
- IgG fraction of polyclonal rabbit antiserum to nuclease from Staphylococcus aureus
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纯化方法
- The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
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免疫原
- Nuclease isolated and purified from Staphylococcus aureus. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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应用备注
- This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice. Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/10,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- IgG protein concentration 10 mg/ml. No foreign proteins added.
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缓冲液
- Purified hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Nuclease
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背景
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
抗原
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