Carbonic Anhydrase 抗体 (Biotin)
The 兔 多克隆 anti-Carbonic Anhydrase antibody is suitable to detect Carbonic Anhydrase in samples from Cow. It has been validated for WB, ELISA, DB, IHC (p) 和 ICC.
产品编号 ABIN459008
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Carbonic Anhydrase 抗体 (Biotin) (ABIN459008)
抗原
Carbonic Anhydrase (CA)
适用
Cow
宿主
兔
克隆类型
多克隆
标记
This Carbonic Anhydrase antibody is conjugated to Biotin
应用范围
Western Blotting (WB), ELISA, Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
-
-
原理
- Rabbit anti Bovine Carbonic anhydrase, conjugated with Biotin
-
特异性
- Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to carbonic anhydrase from Bovine erythrocytes.
-
交叉反应 （详细）
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
-
产品特性
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
-
纯化方法
- Purified IgG fraction from antiserum by ammonium sulphate precipitation and ion exchange chromatography.
-
免疫原
- Carbonic anhydrase isolated and purified from bovine erythrocytes. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
亚型
- IgG
-
-
-
-
应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
-
缓冲液
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
-
-
- Carbonic Anhydrase (CA)
-
别名
- Carbonic Anhydrase
-
UniProt
- P00921
抗原
-
You are here:
