Concanavalin A 抗体 (HRP)
The 山羊 多克隆 anti-Concanavalin A antibody is suitable to detect Concanavalin A in samples from Canavalia ensiformis. It has been validated for WB, ICC, ELISA, IHC (p) 和 DB.
产品编号 ABIN458987
发货至:
中国
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Contact Info
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中国
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Quick Overview for Concanavalin A 抗体 (HRP) (ABIN458987)
抗原
See all Concanavalin A (ConA) 抗体
Concanavalin A (ConA)
适用
Canavalia ensiformis
宿主
山羊
克隆类型
多克隆
标记
This Concanavalin A antibody is conjugated to HRP
应用范围
Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)
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原理
- Goat anti Canavalia Concanavalin A, conjugated with Horseradish peroxidase
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特异性
- Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to concanavalin A from jack bean
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRation of the reactants and the sensitivity of the assay arrangement. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- Tested in immunoelectrophoresis, using different antigen/antibody concentration ratio's the antiserum gives one characteristic precipitin line. In enzyme-immunocytochemical and immunohistochemical staining of concanavalin A in appropriately prepared substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. The entire test system must be free of sodium azide, since it inhibits the enzyme activity.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- The lectin concanavalin A is a cell-agglutinating protein reacting specifically with molecules which contain alpha-D-mannopyranosyl, alpha-D-glucopyranosyl and sterically related residues. At pH 4.5-5.6 it exist as a dimer, above pH 7.0 predominantly as a tetramer. The monomer has a molecular weight of about 52,000. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Horseradish peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- Concanavalin A (ConA)
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别名
- Concanavalin-A
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UniProt
- P02866
抗原
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