Tetramethylrhodamine Isothiocyanate Isomer R 抗体 (HRP)
This 小鼠 单克隆 antibody specifically detects Tetramethylrhodamine Isothiocyanate Isomer R in ELISA 和 WB.
产品编号 ABIN458845
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for Tetramethylrhodamine Isothiocyanate Isomer R 抗体 (HRP) (ABIN458845)
抗原
Tetramethylrhodamine Isothiocyanate Isomer R
宿主
小鼠
克隆类型
单克隆
标记
HRP
应用范围
ELISA, Western Blotting (WB)
克隆位点
NI 308
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特异性
- This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
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产品特性
- Horseradish peroxidase conjugated purified monoclonal mouse antibody to tetramethylrhodamine isothiocyanate isomer R
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免疫原
- Highly purified tetramethylrhodamine isothiocyanate isomer R.
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亚型
- IgG1 kappa
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应用备注
- To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40, in ELISA from 1:100 upwards, in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- IgG concentration is 0.4 mg/ml. Peroxidase/IgG protein molar ratio (E/P) approximately 1.7. No foreign proteins added.
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缓冲液
- Horseradish peroxidase conjugated purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.
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- Tetramethylrhodamine Isothiocyanate Isomer R
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物质类
- Chemical
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背景
- The reactivity of the antiserum is directed to the TRITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting.
抗原
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