FITC 抗体 (Biotin)
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北京 101111
Quick Overview for FITC 抗体 (Biotin) (ABIN458844)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antiserum has not been tested for cross-reactivity with other fluorescent compounds.
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产品特性
- Biotin-conjugated purified monoclonal mouse antibody to fluorescein isothiocyanate isomer 1
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免疫原
- Highly purified fluorescein isothiocyanate isomer 1.
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亚型
- IgG1 kappa
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anti-Fluorescein Isothiocyanate (FITC) antibody
FITC ELISA, WB, IHC, FACS 宿主: 绵羊 Polyclonal unconjugated
anti-Fluorescein Isothiocyanate (FITC) antibodyFITC WB 宿主: 兔 Polyclonal unconjugated
anti-Fluorescein Isothiocyanate (FITC) antibodyFITC 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Fluorescein Isothiocyanate (FITC) antibody (HRP)FITC ELISA, WB, IHC 宿主: 绵羊 Polyclonal HRP
anti-Fluorescein Isothiocyanate (FITC) antibody (Alkaline Phosphatase (AP))FITC ELISA, WB, IHC 宿主: 绵羊 Polyclonal Alkaline Phosphatase (AP)
anti-Fluorescein Isothiocyanate (FITC) antibody (Biotin)FITC ELISA, WB, IHC, FACS 宿主: 绵羊 Polyclonal Biotin
anti-Fluorescein Isothiocyanate (FITC) antibody (PE)FITC ELISA, FACS 宿主: 绵羊 Polyclonal PE
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应用备注
- To enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to FITC. The phenomenon of a weak reaction of a monoclonal antibody is e.g. well known in different analysis of vital peripheral blood mononuclear cells in suspensions by the expression of surface markers. A similar situation exists in solid phase assay systems (ELISA, blotting, DIBA) when used for the identification and /or quantitative determination of minute amounts of soluble specific antigens or antibodies. The sensitivity of the FITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- Physicochemical characteristic IgG concentration is 0.4 mg/ml. No foreign proteins added.
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缓冲液
- Biotin conjugated purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 20 °C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.
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- FITC (Fluorescein Isothiocyanate (FITC))
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别名
- Fluorescein Isothiocyanate Isomer 1
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物质类
- Chemical
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背景
- The reactivity of the antiserum is directed to the FITC molecule as tested in direct binding enzyme immunoassay, ELISA and immunoblotting.
抗原
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