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BETAHYDROXYSTEROID DEHYDROGENASE 抗体 (Biotin) Primary Antibody

适用: Pseudomonas testosteroni IF, ELISA, WB 宿主: 兔 Polyclonal
产品编号 ABIN458821
$408.80
加上运输费 $45.00
1 mL
local_shipping 发货至: 美国
5至7个工作日
  • 抗原
    适用
    Pseudomonas testosteroni
    宿主
    克隆类型
    多克隆
    标记
    Biotin
    应用范围
    Immunofluorescence (IF), ELISA, Western Blotting (WB)
    特异性
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    产品特性
    Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to β-hydroxysteroid dehydrogenase from Pseudomonas testosteroni cells
    纯化方法
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    免疫原
    beta -Hydroxysteroid dehydrogenase isolated and purified from Pseudomonas testosteroni cells. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    亚型
    IgG
  • 抗原
    别名
    beta-hydroxysteroid dehydrogenase
    背景
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
  • 应用备注
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
    限制
    仅限研究用
  • 状态
    Lyophilized
    浓度
    IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.2. No foreign proteins added.
    缓冲液
    Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    储存液
    Without preservative
    储存条件
    4 °C/-20 °C
    储存方法
    The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slow ly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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