BETAHYDROXYSTEROID DEHYDROGENASE 抗体 (Biotin)
This 兔 多克隆 antibody specifically detects BETAHYDROXYSTEROID DEHYDROGENASE in ELISA, WB, IHC (p), ICC 和 DB. It exhibits reactivity toward Pseudomonas testosteroni.
产品编号 ABIN458821
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for BETAHYDROXYSTEROID DEHYDROGENASE 抗体 (Biotin) (ABIN458821)
抗原
BETAHYDROXYSTEROID DEHYDROGENASE
适用
Pseudomonas testosteroni
宿主
兔
克隆类型
多克隆
标记
Biotin
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Dot Blot (DB)
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原理
- Rabbit anti Pseudomonas testosteroni cells b-Hydroxysteroid dehydrogenase, conjugated with Biotin
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特异性
- Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to beta-hydroxysteroid dehydrogenase from Pseudomonas testosteroni cells.
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交叉反应 （详细）
- Cross-reactivities against enzymes of other sources may occur but have not been determined.
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产品特性
- The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
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纯化方法
- Purified IgG fraction from antiserum by ammonium sulphate precipitation and ion exchange chromatography.
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免疫原
- beta-Hydroxybutyrate dehydrogenase isolated and purified from Pseudomonas testosteroni cells. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Biotin-coupled hyperimmune rabbit IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- BETAHYDROXYSTEROID DEHYDROGENASE
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别名
- beta-hydroxysteroid dehydrogenase
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UniProt
- P19871
抗原
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