IgA Secretory Component (Free & Bound) 抗体
The 山羊 多克隆 anti-IgA Secretory Component antibody (ABIN458766) specifically detects IgA Secretory Component in ELISA, WB, IFA 和 DB.
The antibody is reactive with 猴 samples.
产品编号 ABIN458766
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Quick Overview for IgA Secretory Component (Free & Bound) 抗体 (ABIN458766)
抗原
IgA Secretory Component
适用
猴
宿主
山羊
克隆类型
多克隆
标记
非结合性
应用范围
ELISA, Western Blotting (WB), Indirect Immunofluorescence Assay (IFA), Dot Blot (DB)
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抗原表位
- Free & Bound
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原理
- Goat anti Monkey secretory component (free and bound)
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特异性
- Purified IgG fraction of polyclonal Goat antiSerum to rhesus Monkey secretory component, free and bound.
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent of the concentRation of the reactants and he sensitivity of the method. This product may also react with free and bound secretory component in many old-world Monkeys, including Macaca Sylvanus, Cercopithecus, Cynomolgus, Baboon, and also with the secretory component of Chimpanzees. In double radial immunodiffusion a reaction has been observed with free Human secretory component.
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产品特性
- Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with free secretory component. In immunoelectrophoresis against normal monkey milk, using a high electroendosmosis agar plate, free secretory component ism precipitated in the alpha-2 region. This antiserum does not react with other molecular forms of IgA, or with any other secretory or plasma protein and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Secretory component can be present in monkey secretions bound to secretory IgA (sIgA) and in free form. Free monkey secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- IgA Secretory Component
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别名
- Secretory Immunoglobulin A (SIgA)
抗原
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