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Albumin 抗体

This 山羊 多克隆 antibody specifically detects Albumin in WB, ELISA, DB 和 IFA. It exhibits reactivity toward 大鼠.
产品编号 ABIN458740
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Albumin 抗体 (ABIN458740)

抗原

See all Albumin (ALB) 抗体
Albumin (ALB)

适用

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大鼠

宿主

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山羊

克隆类型

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多克隆

标记

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This Albumin antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)
  • 原理

    Goat anti Rat albumin

    特异性

    Purified IgG fraction of polyclonal Goat antiSerum to Rat albumin

    交叉反应

    犬, 马, 人, 猴, 小鼠, Pig

    无交叉反应

    小鸡, 山羊, 绵羊

    交叉反应 (详细)

    Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: cow - Goat - Human + Rabbit ± Chicken - Guinea Pig ++ Monkey + Sheep - Dog + Horse + Mouse ++ Swine +

    产品特性

    The defined antibody specificity is directed to albumin as tested against rat sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin. The cytochemical grade of GARa/Alb/7S allows its use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.

    纯化方法

    Purified IgG fraction from antiserum

    免疫原

    Highly purified albumin isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

    亚型

    IgG
  • 应用备注

    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

    缓冲液

    Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

    储存液

    Without preservative

    储存条件

    4 °C,-20 °C

    储存方法

    The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • 抗原

    Albumin (ALB)

    别名

    Albumin

    UniProt

    P02770

    途径

    Lipid Metabolism
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