Albumin 抗体
Quick Overview for Albumin 抗体 (ABIN458740)
抗原
See all Albumin (ALB) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion against several species sera with the following results: cow - chicken - dog + goat - guinea pig ++ horse + human + monkey + mouse ++ rabbit ± sheep - swine +
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产品特性
- Purified IgG fraction of polyclonal goat antiserum to rat albumin
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纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG (7S) fraction by DEAE-chromatography.
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免疫原
- Highly purified albumin isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Albumin (ALB) (AA 19-609) antibody
ALB 适用: 人 ELISA, WB, IP, IHC (p) 宿主: 小鼠 Monoclonal 1G12-1B3 unconjugated
anti-Albumin (ALB) antibodyALB 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) antibodyALB 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) (C-Term) antibodyALB 适用: 人 WB, IHC, ICC, IF, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) (AA 410-609) antibodyALB 适用: 人 ELISA, WB, IHC, FACS 宿主: 小鼠 Monoclonal 3F6E5 unconjugated
anti-Albumin (ALB) (AA 410-609) antibodyALB 适用: 人, 猴 ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 1F5H7 unconjugated
anti-Albumin (ALB) antibodyALB 适用: 人 ELISA, WB, IF, FACS, Coat 宿主: 小鼠 Monoclonal ALB-2356 unconjugated
anti-Albumin (ALB) (AA 20-255) antibodyALB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) (AA 540-569), (C-Term) antibodyALB 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18676 unconjugated
anti-Albumin (ALB) antibodyALB 适用: 猕猴 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- The cytochemical grade of GARa/Alb/7S allows its use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:10,000.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- IgG protein concentration 10 mg/ml. No foreign proteins added.
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缓冲液
- Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not ref rozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Albumin (ALB)
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别名
- Albumin
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背景
- The defined antibody specificity is directed to albumin as tested against rat sera. In immuno- electrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin.
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途径
- Lipid Metabolism
抗原
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