alpha Fetoprotein 抗体 (FITC)
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北京 101111
Quick Overview for alpha Fetoprotein 抗体 (FITC) (ABIN458738)
抗原
See all alpha Fetoprotein (AFP) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Sheep anti Rat alpha foetoprotein, conjugated with FITC
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特异性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Sheep antiSerum to Rat alpha foetoprotein.
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to mammalian proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in double radial immunodiffusion (Ouchterlony) a precipitation reaction has been observed with Mouse amniotic fluid.
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产品特性
- The reactivity of the antiserum is restricted to alpha-foetoprotein. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against amniotic fluid, rat hepatoma sera and purified alpha-foetoprotein an single characteristic precipitin line is obtained. No reaction is obtained with rat sera of different strains. To identify and measure alpha foetoprotein at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates and to identify and measure alpha-foetoprotein in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Purified rat alpha-foetoprotein is isolated from pooled amniotic fluid and hepatoma serum using a proprietary multiple step method. Several foetoproteins have been demonstrated in rat foetal serum and amniotic fluid. One of the best studied is the alpha-foetoprotein. It can be easily identified immunochemically because of its distinct antigenicity. In electrophoresis its relative mobility is between the albumin and the alpha-1 globulin fractions. Its molecular weight is 65,000, the carbohydrate content is relatively low. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-alpha-Fetoprotein (AFP) (AA 500-609) antibody
AFP 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-alpha-Fetoprotein (AFP) antibodyAFP 适用: 人, 犬, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 2A9 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 399-605) antibodyAFP 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 31-171) antibodyAFP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 409-611) antibodyAFP 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) antibodyAFP 适用: 人 WB, IF, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal A2 unconjugated
anti-alpha-Fetoprotein (AFP) (C-Term) antibodyAFP 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 281-609) antibodyAFP 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 1087CT8-3-5 unconjugated
anti-alpha-Fetoprotein (AFP) (AA 360-609) antibodyAFP 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-alpha-Fetoprotein (AFP) (AA 46-210) antibodyAFP 适用: Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- FITC-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the activity of the conjugate.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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别名
- alpha Foetoprotein
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UniProt
- P02773
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途径
- C21-Steroid Hormone Metabolic Process
抗原
-
