Albumin 抗体 (HRP)
Our Local Distributor
北京 101111
Quick Overview for Albumin 抗体 (HRP) (ABIN458654)
抗原
See all Albumin (ALB) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
原理
- Goat anti Rat albumin, conjugated with Horseradish peroxidase
-
特异性
- Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Rat albumin.
-
交叉反应
- 犬, 马, 人, 猴, 小鼠, Pig
-
无交叉反应
- 小鸡, Cow, 山羊, 绵羊
-
交叉反应 (详细)
- Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: Bovine - Goat - Human + Rabbit ± Chicken - Guinea Pig ++ Mouse ++ Sheep - Dog + Horse + Monkey + Swine + A negative reaction in double radial immunodiffusion does not exclude a weak positive reaction in more sensitive techniques.
-
产品特性
- Tested in immunoelectrophoresis and double radial immunodiffusion against pooled normal rat serum and purified rat albumin. One characteristic precipitin line is obtained against pooled normal rat serum using different antigen/antibody concentration ratio's. Precipitin lines against normal rat serum and purified rat albumin give a reaction of full identity. In enzyme-immunocytochemical and immunohistochemical staining for the detection of albumin, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure albumin in rat serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
-
纯化方法
- Purified IgG fraction from antiserum
-
免疫原
- Purified albumin isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
-
亚型
- IgG
-
-
-
-
应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
- Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
-
储存液
- Without preservative
-
-
- Albumin (ALB)
-
别名
- Albumin
-
UniProt
- P02770
-
途径
- Lipid Metabolism
抗原
-