Albumin 抗体 (FITC)
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北京 101111
Quick Overview for Albumin 抗体 (FITC) (ABIN458653)
抗原
See all Albumin (ALB) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Goat anti Rat albumin, conjugated with FITC
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特异性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Rat albumin.
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交叉反应
- 犬, 马, 人, 猴, 小鼠, Pig
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无交叉反应
- 小鸡, Cow, 山羊, 绵羊
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交叉反应 （详细）
- Inter-species cross-reactivity is a normal feature of antibodies to mammalian Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: Bovine - Goat - Human + Rabbit ± Chicken - Guinea Pig ++ Monkey + Sheep - Dog + Horse + Mouse ++ Swine +
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产品特性
- The defined antibody specificity is directed to albumin as tested against rat sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin. As reagent for the direct detection of albumin in rat cells, tissues and body fluids in immunofluorescence techniques. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from rat serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Albumin (ALB) (AA 19-609) antibody
ALB 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 1G12-1B3 unconjugated
anti-Albumin (ALB) (AA 540-569), (C-Term) antibodyALB 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB18676 unconjugated
anti-Albumin (ALB) (AA 410-609) antibodyALB 适用: 人, 猴 ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 1F5H7 unconjugated
anti-Albumin (ALB) (AA 410-609) antibodyALB 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 3F6E5 unconjugated
anti-Albumin (ALB) (C-Term) antibodyALB 适用: 人 WB, IHC, ICC, IF, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) antibodyALB 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) antibodyALB 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) antibodyALB 适用: 人 WB, ELISA, IF, FACS 宿主: 小鼠 Monoclonal ALB-2356 unconjugated
anti-Albumin (ALB) (AA 20-255) antibodyALB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Albumin (ALB) antibodyALB 适用: 人 WB, ELISA, IHC, IF 宿主: 小鼠 Monoclonal ALB-2355 unconjugated
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应用备注
- ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- Albumin (ALB)
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别名
- Albumin
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UniProt
- P02770
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途径
- Lipid Metabolism
抗原
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