Lactoferrin 抗体 (FITC)
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- 抗原 See all Lactoferrin (LTF) 抗体
- Lactoferrin (LTF) (Lactotransferrin (LTF))
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适用
- 猴
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Lactoferrin antibody is conjugated to FITC
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应用范围
- ELISA
- 特异性
- The antiserum does not cross-react with any other monkey plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail , however a strong cross-reaction with lactoferrin in human milk has been observed.
- 产品特性
- Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to monkey lactoferrin
- 纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized fractions of monkey serum and lactoferrin-depleted monkey milk as required, to eliminate antibodies reacting with other monkey serum or milk proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.
- 免疫原
- Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight anti-microbial action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from monkey milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 亚型
- IgG
- Top Product
- Discover our top product LTF Primary Antibody
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- 应用备注
- As reagent for the direct detection of lactoferrin in monkey cells, tissues and body fluids in immunofluores-cence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- IgG protein concentration 10 mg/ml. Fluorescein/IgG protein molar ratio (F/P) approximately 1.6. No foreign proteins added.
- 缓冲液
- Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)
- 储存液
- Without preservative
- 储存条件
- 4 °C
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- 抗原
- Lactoferrin (LTF) (Lactotransferrin (LTF))
- 别名
- Lactoferrin (LTF 产品)
- 别名
- LTF antibody, GIG12 antibody, HLF2 antibody, LF antibody, Lf antibody, Csp82 antibody, MMS10R antibody, Ms10r antibody, PLF antibody, TFEW antibody, conalbumin antibody, lactotransferrin antibody, transferrin antibody, transferrin (ovotransferrin) antibody, LTF antibody, CpipJ_CPIJ000114 antibody, TF antibody, Ltf antibody
- 背景
- Tested in immunoelectrophoresis against rhesus monkey milk a single precipitin line is obtained. The antiserum does not react with any other protein component of monkey serum or plasma
- 途径
- Transition Metal Ion Homeostasis
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