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Lactoferrin 抗体

The 山羊 多克隆 anti-Lactoferrin antibody (ABIN458259) specifically detects Lactoferrin in IEP, RID 和 ODD. The antibody is reactive with 猴 samples.
产品编号 ABIN458259
发货至: 中国
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Quick Overview for Lactoferrin 抗体 (ABIN458259)

抗原

See all Lactoferrin (LTF) 抗体
Lactoferrin (LTF) (Lactotransferrin (LTF))

适用

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猴

宿主

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山羊

克隆类型

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多克隆

标记

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This Lactoferrin antibody is un-conjugated

应用范围

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Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID), Ouchterlony Double Diffusion (ODD)
  • 原理

    Goat anti Monkey lactoferrin

    特异性

    Precipitating polyclonal Goat antiSerum to Monkey lactoferrin.

    交叉反应 (详细)

    The antiSerum does not cross-react with any other Monkey plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

    产品特性

    In immunoelectrophoresis against monkey milk a single precipitin line is obtained. The antiserum does not react with any other protein component of monkey serum or plasma. In precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion Mancini, Ouchterlony)and electroimmunodiffusion (Laurell) for the identification and quantitative determination of lactoferrin in body fluids.

    纯化方法

    Delipidated, heat inactivated, lyophilized, stable whole antiserum

    免疫原

    Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight antimicrobal action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears. The immunogen has been isolated from rhesus monkey milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

    亚型

    IgG
  • 应用备注

    Precipitation assays. In immunoelectrophoresis use 2 μL monkey serum against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter center well and 10 μL of the antiserum in 3 mm diameter peripheral wells. In single radial immunodiffusion use 1 % antiserum in the gel.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

    缓冲液

    Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.

    储存液

    Without preservative

    储存条件

    4 °C,-20 °C

    储存方法

    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
  • 抗原

    Lactoferrin (LTF) (Lactotransferrin (LTF))

    别名

    Lactoferrin

    途径

    Transition Metal Ion Homeostasis
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