LALBA 抗体
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北京 101111
Quick Overview for LALBA 抗体 (ABIN458233)
抗原
See all LALBA 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Rabbit anti Human lactalbumin
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特异性
- IgG fraction of polyclonal Rabbit antiSerum to Human alpha-lactalbumin.
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交叉反应 (详细)
- Homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum has not been tested for cross-reactivity.
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产品特性
- The defined antibody specificity is directed to alpha-lactalbumin as tested against human milk. Its presence can also be demonstrated in udder tissues. When tested in immunoelectrophoresis and double radial immunodiffusion against serum no reaction is obtained. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Highly purified alpha-lactalbumin isolated from human milk. Alpha-lactalbumin is one of the major proteins in the alpha region after immunoelectrophoresis of human milk. It has been implicated as a component of the enzyme lactose synthetase which catalyzes the formation of lactose from uridine diphosphate galactose and glucose. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.
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缓冲液
- Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
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- LALBA (Lactalbumin, alpha- (LALBA))
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别名
- alpha-Lactalbumin
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UniProt
- P00709
抗原
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