LYZ 抗体 (AA 5-220)
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北京 101111
Quick Overview for LYZ 抗体 (AA 5-220) (ABIN458231)
抗原
See all LYZ 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 5-220
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原理
- Rabbit anti Human lysozyme
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特异性
- IgG fraction of polyclonal Rabbit antiSerum to Human lysozyme.
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交叉反应 （详细）
- The antiSerum does not cross-react with any other component of Human plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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产品特性
- The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. The cytochemical grade allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
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纯化方法
- Purified IgG fraction from antiserum
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免疫原
- Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low, in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme.Highly purified recombinant human lysozyme was used as immunogen. Freund's complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-Lysozyme (LYZ) (AA 1-100) antibody
LYZ 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人 ICC, FACS, MS 宿主: 小鼠 Monoclonal LZ598-10G9 unconjugated
anti-Lysozyme (LYZ) (AA 19-148) antibodyLYZ 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) antibodyLYZ 适用: 小鼠, 大鼠 WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 18-147) antibodyLYZ 适用: Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 24-144) antibodyLYZ 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) (AA 19-148) antibodyLYZ 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lysozyme (LYZ) antibody (FITC)LYZ 适用: 人 ICFC 宿主: 小鼠 Monoclonal LZ598-10G9 FITC
anti-Lysozyme (LYZ) antibody (PE)LYZ 适用: 人 ICFC 宿主: 小鼠 Monoclonal LZ598-10G9 PE
anti-Lysozyme (LYZ) antibodyLYZ 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1C9 unconjugated
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应用备注
- Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
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储存条件
- 4 °C,-20 °C
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储存方法
- The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C.
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- LYZ (Lysozyme (LYZ))
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别名
- Lysozyme
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UniProt
- P61626
抗原
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